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NAC是植物特有的一类转录因子,在植物生长发育和抗逆过程中有重要作用。本研究从毛白杨中克隆出一个NAC转录因子基因,并根据同源性分析将其命名为Pto NAC157,开放阅读框为942 bp,能编码一个由313个氨基酸残基组成的蛋白质,预测蛋白分子量为35.5 kDa,等电点为7.22。氨基酸同源性分析显示,该蛋白N-端具有保守的NAC结构域。实时荧光定量PCR结果表明:PtoNAC157在毛白杨幼根、茎和叶中均有表达,在茎中的表达量最高。PtoNAC157响应低温、NaCl(300mmol·L-1)、干旱和ABA(200μmol·L-1)胁迫。由此推测该基因在林木次生生长及响应胁迫信号转导过程中发挥作用。
NAC is a plant-specific transcription factor that plays an important role in plant growth and development. In this study, we cloned a NAC transcription factor gene from Populus tomentosa and named it Pto NAC157 according to homology analysis. The open reading frame is 942 bp, which encodes a protein consisting of 313 amino acid residues and predicted the molecular weight 35.5 kDa and isoelectric point of 7.22. Amino acid homology analysis showed that the N-terminal of the protein has a conserved NAC domain. Real-time PCR results showed that PtoNAC157 was expressed in root, stem and leaf of Populus tomentosa and highest in stem. PtoNAC157 responded to low temperature, NaCl (300mmol·L-1), drought and ABA (200μmol·L-1) stress. Therefore, it is speculated that this gene plays a role in secondary growth of forest trees and in response to stress signal transduction.