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目的 :了解HLC小鼠血液学指标及T淋巴细胞及其亚群 ,提供HLC小鼠的生物学数据。方法 :分别对HLC小鼠、无毛小鼠及昆明小鼠进行RBC、WBC、BPC、Hb测定及白细胞分类计数。α 醋酸萘酯酶法染色观察其T淋巴细胞及其亚群。结果 :HLC小鼠RBC、WBC、BPC、Hb与无毛小鼠及昆明小鼠相比 ,差异无统计学意义 ,3种小鼠的白细胞分类计数基本一致。HLC小鼠T淋巴细胞总数、T辅助细胞及T抑制细胞与昆明小鼠及无毛小鼠比较 ,差异均无统计学意义 (P >0 .0 5)。结论 :HLC小鼠血液学指标与昆明小鼠及无毛小鼠相一致 ,T淋巴细胞及其亚群趋于正常 ,可作为新的小鼠近交系应用于医学生物学研究
OBJECTIVE: To understand the hematological parameters and T lymphocytes and their subsets in HLC mice and provide the biological data of HLC mice. Methods: RBC, WBC, BPC, Hb and white blood cell count were counted in HLC mice, hairless mice and Kunming mice respectively. α naphthyl acetate enzymatic staining of T lymphocytes and their subgroups. Results: There was no significant difference in RBC, WBC, BPC, Hb between HLC mice and hairless mice and Kunming mice. The leukocyte counts of three kinds of mice were basically the same. There was no significant difference in the total number of T lymphocytes, T helper cells and T suppressor cells between HLC mice and Kunming mice and hairless mice (P> 0.05). Conclusion: The hematological indexes of HLC mice are consistent with those of Kunming mice and hairless mice. The T lymphocytes and their subpopulations tend to be normal and can be used as new mouse inbred lines in medical biology research