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本文在激光共聚焦扫描显微镜下观察了内源性 5 -HT终末、 、 脑神经初级传入终末在孤束核内与向臂旁核投射神经元之间的联系。分别向臂旁外侧核微量注射四甲基罗丹明 , 、 脑神经干内微量注射生物素化葡聚糖胺 ,以免疫荧光和荧光组织化学方法显示 5 -HT、内脏神经初级传入终末和孤束核内的四甲基罗丹明逆行标记神经元。结果发现 ,大量罗丹明逆行标记细胞 (红色 )主要位于注射侧孤束核的的内侧、小细胞亚核及连合核。同部位也同时观察到 5 -HT能阳性终末 (蓝色 )和内脏神经初级传入终末 (绿色 )。两种终末的终扣和膨体紧靠逆行标记神经元的胞体和树突或与其重叠 ,这些可能是突触连接部位的接触点。本文结果提示 :中枢内的下行性 5 -HT纤维可能直接作用于孤束核内向臂旁核投射的神经元且通过与内脏神经传入终末在此处的汇聚 ,而产生新的神经效应
In this paper, the endogenous 5-HT terminal was observed under confocal laser scanning microscopy, and the relationship between primary afferent terminals of neurons in the solitary nucleus and neurons projecting to the parabrachial nucleus. Tetramethylrhodamine was injected into the lateral para-arm nucleus and microinjected with biotinylated dextran amine. The immunofluorescence and fluorescence histochemical method showed that 5-HT, primary afferent end of visceral nerve and Tetramethylrhodamine retrogradely labeled neurons in nucleus tractus solitarius. The results showed that a large number of rhodamine retrogradely labeled cells (red) were mainly located on the medial side of the nucleus tractus solitarius, the subtypes of small cells and the commissural nucleus. The same site also observed 5-HT positive terminal (blue) and visceral nerve primary afferent terminal (green). The terminal and bulge of both terminals mark or overlap closely with retrogradely labeled cell bodies and dendrites of neurons, which may be the point of contact for the synaptic junction. Our results suggest that descending 5-HT fibers in the central nerve may act directly on neurons projecting into the parabrachial nucleus in the nucleus tractus solitarius and create new neuronal effects by converging with the visceral nerve afferent terminals here