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目的:建立绞股蓝药材中芦丁、人参皂苷Rb1和槲皮素的含量测定方法。方法:TSK ODS-100V(4.6 mm×250mm,5μm)色谱柱;流动相乙腈-0.1%磷酸(30∶70),流速1.0 mL.min-1,柱温30℃,进样量10μL,检测波长(0～14 min,257nm;14～18 min,203 nm;18～30 min,257 nm)。结果:芦丁、人参皂苷Rb1和槲皮素分别在0.171 2～1.284 0μg、0.828 0～6.210 0μg,0.204 8～1.525 7μg线性关系良好,回归方程分别为Y=732 927.570 1 X+1 523.016 4,r=0.9998、Y=158 991.787 4X+307.545 6,r=0.999 9,Y=745 728.515 6 X+2 101.166 8(r=0.999 8),平均加样回收率分别为97.33%(RSD 2.02%),98.61%(RSD 1.70%),98.38%(RSD 2.59%)。结论:所建立的方法准确性高,重复性好,可作为绞股蓝药材的质量控制方法。
Objective: To establish a method for the determination of rutin, ginsenoside Rb1 and quercetin in Gynostemma Pentaphyllum. The mobile phase was acetonitrile-0.1% phosphoric acid (30:70), the flow rate was 1.0 mL · min-1. The column temperature was 30 ℃ and the injection volume was 10 μL. The detection wavelength (0-14 min, 257 nm; 14-18 min, 203 nm; 18-30 min, 257 nm). Results: The linear relationships between rutin, ginsenoside Rb1 and quercetin were good at 0.171 2 ~ 1.284 0μg, 0.828 0 ~ 6.210 0μg and 0.204 8 ~ 1.525 7μg respectively. The regression equations were Y = 732 927.570 1 X + 1 523.016 4, r = 0.9998, Y = 158991.787 4X + 307.545 6, r = 0.999 9, Y = 745 728.515 6X +2 101.1668 (r = 0.999 8), and the average recoveries were 97.33% (RSD 2.02%), 98.61% (RSD 1.70%), 98.38% (RSD 2.59%). Conclusion: The established method has high accuracy and repeatability and can be used as quality control method of Gynostemma.