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以多裂叶荆芥无菌幼苗根、茎段、叶片等为外植体,进行不定芽离体再生,建立了多裂叶荆芥离体快速繁殖体系,研究了不同外植体和附加不同外源激素配比对不定芽离体再生的影响。结果表明:多裂叶荆芥离体再生的最佳外植体为带有叶腋的茎段,诱导不定芽再生的适宜培养基为MS+0.2mg/L NAA+1.0mg/L TDZ,每个外植体产生不定芽数平均为18个;不定芽增殖培养每4周为1个周期,理论年繁殖系数可达1×1812;当不定芽生长至3~4cm时移入生根培养基诱导生根;适宜的生根培养基为MS+0.2mg/L NAA,移栽成活率为53.85%。
The explants of roots, stem segments and leaves of sterile seedlings of Schizonepeta tenuiflora were used as explants for in vitro regeneration of adventitious buds, and the rapid propagation system was established in vitro and the effect of different exogenous hormones Effect of Adventitious Buds on Regeneration in vitro. The results showed that the best medium for explants regenerated from Schizonepeta tenuiflora was leaf stem with leaf axils, and the suitable medium for inducing adventitious shoots regeneration was MS + 0.2mg / L NAA + 1.0mg / L TDZ. Each explant The number of adventitious buds produced was 18 on average. Adventitious buds multiplication culture was 1 cycle every 4 weeks, and the multiplication coefficient of theoretical adventitious buds could reach 1 × 1812. When the adventitious buds grew to 3 ~ 4 cm, they were transferred into rooting medium to induce rooting. The rooting medium was MS + 0.2mg / L NAA, the survival rate of transplanting was 53.85%.