论文部分内容阅读
目的 调查江西省耐多药结核分枝杆菌MIRU-VNTR分子特征及传播情况. 方法 分离自江西地区的耐多药结核分枝杆菌131株,采用15位点MIRU-VNTR进行RD105片段缺乏(北京基因行)检测;进行分型,计算等位点差异、分辨率、成簇率以及近期感染率的估算值. 结果 江西地区人感染耐多药结核分枝杆菌中北京基因型占 83.21％.分离株结核分枝杆菌15位点MIRU-VNTR的HGI值为0.9947,北京家族菌为0.9923.131株菌共分为11个基因簇和98个孤立基因型,成簇率为25.19％,近期感染率估计值为16.79％;北京家族株成簇率为28.44％,近期感染率估计值为19.27％. 结论 15位点MIRU-VNTR对包括北京家族株结核分枝杆菌的分辨率高,该方法适合于江西地区耐多药结核分枝杆菌的基因分型.江西省耐多药结核病的流行主要为内源性复燃,但近期传播仍不能忽视.“,”Objectives To determine the epidemiological and molecular characteristics of multi-drug-resistant Mycobacterium tuberculosis (MDR-TB)in Jiangxi Province using mycobacterial interspersed repetitive unit-variable number tandem repeat typing(MIRU-VNTR). Methods Strains with RD105deletion were identified as Beijing family isolates. MDR-TB was genotyped using 15-locus MIRU-VNTR.Allelic diversity and the discriminatory index were calculated,and the cumulative clustering rate and minimum infection rate were estimated. Results The proportion of patients infected with Beijing family isolates of MDR-TB was 83.21％.The Hunter-Gaston discriminatory index for 15loci was 0.9947, and that for Beijing family isolates was 0.9923.Eleven clusters and 98unique genotypes were identified,and the clustering rate was 25.19％.The smallest estimated proportion of recent transmission was 16.79％.The clustering rate for Beijing family isolates was 28.44％and the smallest estimated proportion of recent transmission was 19.27％. Conclusion Fifteen-locus MIRU-VNTR had great power to differentiate Beijing family isolates,and it was suitable for genotyping MDR-TB isolates in Jiangxi Province.Outbreaks of MDR-TB in Jiangxi are mainly due to reactivation,but recent transmission has also played a role.