应用电镜及同位素掺入试验,研究α蒎烯的抗真菌作用及其机制。结果表明:α蒎烯对白念珠菌有明显的抑菌和杀菌作用。电镜下,α蒎烯作用后的白念珠菌,其形态和超微结构发生明显的变化,菌细胞壁及细胞膜破裂,菌体内容物漏出,菌细胞裂解后成块状物;DNA,RNA 及胞壁中多糖和胞膜的麦角固醇合成受到明显的抑制。提示α蒎烯抗真菌的作用机制是通过抑制真菌的DNA,RNA,多糖及麦角固醇的生物合成所致 The application of electron microscopy and isotope incorporation test to study the anti-fungal activity of α-pinene and its mechanism. The results showed that α-pinene had obvious antibacterial and bacteriostasis effects on Candida albicans. Under the electron microscope, the morphology and ultrastructure of Candida albicans after α-pinene treatment changed obviously, the cell wall and cell membrane ruptured, the content of bacteria leaked, and the bacterial cells became lumps after lysis; DNA, RNA and The synthesis of ergosterol in cell wall polysaccharides and membranes was significantly inhibited. Tip α-pinene anti-fungal mechanism of action is through the inhibition of fungal DNA, RNA, polysaccharides and ergosterol biosynthesis caused