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目的:研究脑内胆碱能系统功能在衰老过程中的变化,以探讨艾灸延缓衰老的作用机制.方法:以自然老龄大鼠为衰老模型,设以青年组、老年组和老年艾灸组:老年艾灸组则用直径约0.8cm的艾条温和灸“百会”、“肾俞”穴;利用HPLC分析技术观察乙酰胆碱(ACh)、胆碱乙酰转移酶(ChAT)、胆碱脂酶(AChE)的含量及活性.结果:通过8个疗程的艾灸治疗,艾灸组ACh的含量及ChAT的活性有所增高,而AChE活性则用所降纸.结论:说明艾灸能通过对中枢胆碱能损害的修复作用,而达到延缓脑老化的功效.“,”Objective To observe the effect of moxibustion of Bǎihuì(百会GV20)and Shènshū(肾俞BL23)on acetylcholine(ACh)content and choline acetyl transferase(ChAT)activity in the brain of aging rats so as to investigate its underlying mechanism in delaying brain aging in the rat. Methods Thirty Wistar rats including 10 young rats(young group)and 20 aged rats that were divided into aging group and moxibustion group evenly，were used in this study．For rats of moxibustion group，moxibustion was applied to Bǎihuì(百会GV20)and Shènshū (肾俞BL23) for 10 min，once a day，with 5 days being a course of treatment，8 courses altogether. At the end of the experiments, the rats anesthetized with 6%chloral hydrate were decap-itated for taking brain tissue, then ACh content, ChAT and acetylcholinesterase(AChE)activity were detected by using high performance liquid chromatography(HPLC). Results Compared with young rat group, Ach contents of basal ganglia, hippocampus and cerebral cortex tissues in aging group and moxibustion group all decreased significantly(P＜0.05, 0.01), while compared with aging group, ACh contents in the 3 cerebralregions in moxibustion group all increased considerably(P＜0.01). In comparison with young group, both ChAT activity and AChE activity of the brain tissue in aging group and moxibustion group lowered significantly (P＜0.05, 0.01);comparison between aging group and moxibustion group showed that ChAT activity of the later group increased evidently(P＜0.05)while AChE activity in moxibustion group decreased considerably (P＜0.01), displaying that moxibustion could potentiate ChAT activity and further lower AChE activity of the brain in aged rats．Conclusion Moxibustion of Bǎihuì(百会GV20)and Sèhnshū(肾俞BL23)has effects in raising ACh contents and lowering ChAT and AChE activity, which may contribute to its efficacies in repairing the injured central cholinergic nerve system and delaying the aging process in the aged rats.