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为明确东农冬麦1号耐寒越冬的生理机制,对不同温度条件下的东农冬麦1号幼苗分蘖节进行GC-TOF/MS代谢组学检测,并采用SIMCA软件对获得的代谢组学数据进行多元变量模式识别分析。结果表明,对温度变化反应明显的代谢产物有54个,对其进行KEGG分析发现,变化显著的代谢通路为:精氨酸和脯氨酸代谢;甘氨酸、丝氨酸和苏氨酸代谢;丙氨酸、天冬氨酸和谷氨酸盐代谢;丙酮酸代谢;糖酵解和糖异生以及肌醇磷酸盐代谢。分析不同温度下以上代谢通路中的差异化合物发现,室内培养组中,分蘖节中的γ-氨基丁酸、蔗糖-6-磷酸以及尿素等含量显著提高,使植株保持了较高的氮素含量,揭示了较高温度下(室内)小麦出现徒长的原因。研究结果可为进一步研究冬小麦越冬的生理特性提供新的研究思路。
In order to clarify the physiological mechanism of Dongnong Dongmai 1’s cold-tolerance overwintering, GC-TOF / MS metabolomics of Dongnong Dongmai 1’s tillering node under different temperature conditions were carried out. Simulated metabolomics Data multivariate pattern recognition analysis. The results showed that there were 54 metabolites that had obvious reaction to temperature change. KEGG analysis showed that the metabolic pathways were: arginine and proline metabolism; glycine, serine and threonine metabolism; alanine , Aspartate and glutamate metabolism; pyruvate metabolism; glycolysis and gluconeogenesis as well as inositol phosphate metabolism. Analysis of the differential compounds in the above metabolic pathways at different temperatures found that the content of γ-aminobutyric acid, sucrose-6-phosphate and urea in the tillering knot significantly increased in the indoor culture group, which kept the plant higher nitrogen content , Revealing the reason why the wheat is lengthened at higher temperatures (indoors). The results can provide new research ideas for further study on the physiological characteristics of winter wheat overwintering.