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探讨龙眼果肉醇提物的石油醚相(LP)和水相(LW)对小鼠学习记忆功能的改善效果及作用机制。以SAMP8小鼠为老年痴呆模型,采用Morris水迷宫观察小鼠的学习记忆能力;检测小鼠血清中一氧化氮(NO)、丙二醛(MDA)和谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力;Western blot法检测小鼠脑组织中β-淀粉样蛋白(Aβ1-42)等相关蛋白(淀粉样前体蛋白(APP)、β-淀粉样前体蛋白裂解酶1(BACE1))的表达水平。研究发现,与模型组相比,LP和LW能显著改善痴呆小鼠的学习记忆功能,分别不同程度地降低其血清中NO和MDA含量以及脑组织中Aβ1-42、APP和BACE1的蛋白表达,提高其血清中SOD和GSH-Px活力。以上结果表明LP和LW可能通过提高小鼠机体的抗氧化能力和抑制其脑组织中Aβ等相关蛋白的表达的共同作用来改善痴呆小鼠的学习记忆功能,具有预防老年痴呆症的潜在功效。
To investigate the effect and mechanism of petroleum ether phase (LP) and aqueous phase (LW) of ethanol extract of longan on the learning and memory of mice. Using SAMP8 mice as model of Alzheimer’s disease, Morris water maze was used to observe the learning and memory ability of mice. The content of serum nitric oxide (NO), malondialdehyde (MDA) and glutathione (GSH) (SOD) and glutathione peroxidase (GSH-Px) were detected by Western blotting. Apoptosis of amyloid precursor protein (Aβ1-42) and other related proteins Protein (APP), beta-amyloid precursor protein lyase 1 (BACE1)). The study found that compared with the model group, LP and LW can significantly improve learning and memory in mice with dementia, respectively, to varying degrees, reduce the content of NO and MDA in serum and the expression of Aβ1-42, APP and BACE1 in brain tissue, Improve their serum SOD and GSH-Px activity. The above results indicate that LP and LW may improve the learning and memory function of dementia mice by increasing the antioxidant capacity of mice and inhibiting the expression of Aβ and other related proteins in their brain tissue and have the potential efficacy of preventing Alzheimer’s disease.