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目的观察大鼠骨髓间充质干细胞骨向诱导分化来源的成骨细胞在张应力刺激下破骨细胞分化因子(osteo-clast differentiation factor,ODF)基因表达的变化,探讨正畸牙移动骨改建过程中成骨细胞调控破骨细胞分化成熟的机制。方法分离培养大鼠股骨骨髓间充质干细胞来源的成骨细胞,采用膜式动态张应力加载体系按张应力大小及作用方式不同分为静态1、3、5 kPa组,动态3、5 kPa组(频率0.017 Hz)及对照不加力组6个实验组和按不同作用时间分为0、3、6、9、12、24和48 h 7个时间段进行体外细胞加力,RT-PCR技术检测不同强度、不同性质、不同作用时间张应力对成骨细胞ODF mRNA表达的影响。结果张应力刺激抑制成骨细胞ODF的表达,静态张应力的抑制效应弱;抑制作用与刺激强度无明显关系;ODF mRNA在动态张应力作用6 h后表达逐渐下降,9 h后显著下降,之后维持在一较低水平,48 h最低,具有时效性。结论机械张应力刺激通过抑制成骨细胞ODF mRNA的表达限制破骨细胞的分化成熟。
OBJECTIVE: To observe the changes of osteo-clast differentiation factor (ODF) gene expression in bone-derived osteoblasts derived from rat bone marrow-derived mesenchymal stem cells under tensile stress, Osteoblasts modulate the mechanism of osteoclast differentiation and maturation. Methods Osteoblasts derived from rat femoral bone marrow mesenchymal stem cells were isolated and cultured. The membrane dynamic tensile stress loading system was divided into static 1,3,5 kPa group and dynamic 3,5 kPa group according to the size and mode of action. (Frequency 0.017 Hz) and control group were not added force six experimental groups and divided into different duration of action 0,3,6,9,12,24 and 48 h seven time periods in vitro cell by force, RT-PCR technology The effects of tensile stress of different intensity, different properties and different time on ODF mRNA expression in osteoblasts were detected. Results Tensile stress inhibited the ODF expression of osteoblasts, and the inhibition effect of static tensile stress was weak. There was no significant relationship between inhibition and stimulation intensity. ODF mRNA expression gradually decreased after 6 h of dynamic tensile stress and decreased significantly after 9 h Maintained at a low level, 48 h minimum, with timeliness. Conclusion Mechanical tensile stress can inhibit osteoclast differentiation and maturation by inhibiting the expression of ODF mRNA in osteoblasts.