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在120 mg/kg单次药饵投喂和首剂量投喂复方磺胺嘧啶(磺胺嘧啶∶甲氧苄啶=5∶1)120 mg/kg后,再按60 mg/kg的剂量连续5 d投喂条件下,采用HPLC法研究了复方磺胺嘧啶在吉富罗非鱼(Oreochromis niloticus)体内的代谢消除规律。结果显示,单次给药下磺胺嘧啶和甲氧苄啶在罗非鱼血浆、肌肉、肾脏和肝脏中的消除半衰期(t1/2ke)分别为16.82和5.99,23.40和13.49,13.43和6.53,15.9和11.02 h,甲氧苄啶在各组织中的消除均快于磺胺嘧啶。给药24 h内,药物在各组织中的比值(SD∶TMP)均在1~40∶1(除了肾脏4 h采样点为0.71)的理想抑菌浓度范围内,提示5∶1给药比例在罗非鱼上使用能够达到理想的体内抑菌效果。血浆较组织中具有更高的比值,这与甲氧苄啶在体内的快速分布特性一致。连续给药情况下,磺胺嘧啶的浓度较单次给药情况下显著提高,甲氧苄啶的浓度较单次给药并未显著提高,这可能与甲氧苄啶较快的代谢速率及体内快速分布有关。参考中华人民共和国农业部NY5070—2002号公告中对水产品中磺胺类药物总量最大残留限量(MRL)为0.1 mg/kg,甲氧苄啶MRL为0.05 mg/kg的规定,单次给药和连续给药情况下,计算的休药期结果一致,建议本实验条件下的休药期不低于12 d。
After a single bait dose of 120 mg / kg and a first dose of 120 mg / kg of compound sulfadiazine (sulfadiazine: trimethoprim = 5: 1) At the same time, the metabolism of compound sulfadiazine in Oreochromis niloticus was studied by HPLC. The results showed that the elimination half-life (t1 / 2ke) of sulfadiazine and trimethoprim in plasma, muscle, kidney and liver of tilapia were 16.82 and 5.99, 23.40 and 13.49, 13.43 and 6.53, 15.9 And 11.02 h, trimethoprim elimination in all tissues were faster than sulfadiazine. Within 24 h after drug administration, the ratio of drug to each tissue (SD: TMP) was in the range of 1: 40: 1 (except for the 4 h sampling point of the kidney) Used in tilapia to achieve the desired effect of antibacterial in vivo. Plasma has a higher ratio than tissue, which is in line with the rapid distribution of trimethoprim in the body. In the case of continuous administration, the concentration of sulfadiazine significantly increased compared with the single administration, and the concentration of trimethoprim was not significantly higher than that of single administration, which may be related to the faster metabolic rate of trimethoprim and the in vivo Fast distribution related. Reference to the People’s Republic of China Ministry of Agriculture notice of NY5070-2002 aquatic products in the maximum residue limit of sulfonamides (MRL) of 0.1 mg / kg, trimethoprim MRL of 0.05 mg / kg provisions of a single administration In the case of continuous administration, the calculated resting drug results were consistent, suggesting that the withdrawal period should not be less than 12 days under the experimental conditions.