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目的探讨白血病骨髓基质对白血病形成的作用机制。方法白血病患者和正常人的骨髓单个核细胞行基质细胞培养,动态观察贴壁时间、小丛形成时间、集落形成时间、融合形成时间等生长特性,计数CFU-F集落,检测基质细胞VCAM-1、Fn及培养上清液中的IL-6、TNF-α含量。结果急性白血病骨髓基质细胞生长延迟,贴壁形成时间、小丛形成时间、集落形成时间和融合形成时间均明显晚于正常骨髓基质组(P<0.05);CFU-F计数和VCAM-1表达水平均明显低于正常对照组(P<0.05),Fn与正常对照组无显著性差异(P>0.05);骨髓基质细胞培养上清液中IL-6、TNF-α含量明显高于正常对照组(P<0.05)。结论急性白血病存在造血微环境异常。
Objective To investigate the mechanism of leukemia bone marrow stromal cells on the formation of leukemia. Methods The bone marrow mononuclear cells of leukemia patients and normal people were cultured in stroma cells. The growth characteristics of adherent cells, formation time of small clusters, colony forming time and the time of fusion formation were dynamically observed. CFU-F colonies were counted to detect the expression of VCAM-1 , Fn and culture supernatant IL-6, TNF-α content. Results The leukemia bone marrow stromal cells had delayed growth, adherent formation time, small plexus formation time, colony formation time and the time of fusion formation were significantly later than those in normal bone marrow stromal cells (P <0.05). CFU-F count and VCAM-1 expression (P <0.05). There was no significant difference between Fn and normal control group (P> 0.05). The contents of IL-6 and TNF-α in supernatant of bone marrow stromal cells were significantly higher than those in normal control group (P <0.05). Conclusion There is abnormal hematopoietic microenvironment in acute leukemia.