论文部分内容阅读
以农业废弃物稻草和猪粪为发酵原料,首次采用纤维素降解复合菌系对稻草和猪粪混合物进行生物处理,通过考察不同碳氮比(25∶1、30∶1、35∶1和40∶1)条件下稻草和猪粪混合物生物预处理的发酵特征及后续的产甲烷能力,探讨了碳氮比对稻草和猪粪的协同生物预处理及厌氧消化效果的影响.结果表明,控制碳氮比为30∶1、料水比为11%时,稻草和猪粪混合物经纤维素降解复合菌系于55℃预处理30 h后其厌氧消化效果最佳.在此条件下,稻草和猪粪降解液中滤纸酶活和羧甲基纤维素酶酶活分别达到了2.18和2.31 IU,其失重率高达41.69%;随后经厌氧发酵后其甲烷产率和产甲烷速率分别可达318.14 m L·g-1(以VS计)和10.61 m L·d-1·g-1(以VS计),且总量为9.9 g的稻草和猪粪混合物的总甲烷产量可达1948 m L,上述结果相对于未经生物预处理的对照组均提高了38%.本研究结果可进一步为其它种类的农作物秸秆和畜禽粪便的高效资源化利用提供理论支撑,展现出了巨大的应用潜力.
Using straw and pig manure as raw materials for fermentation, the mixture of straw and pig manure was first treated by cellulolytic complex strains. The effects of different C / N ratio (25:1, 30:1, 35:1 and 40% : 1), the biological characteristics of pretreatment of rice straw and pig manure mixture and subsequent methanogenic ability were studied, and the effects of C / N ratio on the synergistic biological pretreatment and anaerobic digestion of rice straw and pig manure were discussed. The results showed that the control When the C / N ratio was 30:1 and the water / water ratio was 11%, the anaerobic digestion of rice straw and swine manure mixture was the best under the condition of pretreatment at 55 ℃ for 30 h.In this condition, straw And pig manure degradation liquid filter paper activity and carboxymethyl cellulose enzyme activity reached 2.18 and 2.31 IU respectively, the weight loss rate as high as 41.69%; followed by anaerobic fermentation methane yield and methanogenic rate up to 318.14 m L · g-1 (in VS) and 10.61 m L · d-1 · g-1 (in VS), and the total methane output of straw and pig manure mixture with total amount of 9.9 g reached 1948 m L, compared with the control group without biological pretreatment were increased by 38% .The results of this study can be further for other species Straw and manure efficient resource utilization to provide theoretical support, showing great potential.