论文部分内容阅读
目的探讨B19病毒vplu蛋白是否影响心脏发育及相关机制。方法对不同孕龄母鼠注射B19病毒vplu蛋白,采集胎鼠心脏组织标本,通过光镜、电镜、免疫组织化学、TUNEL和原位杂交技术观察心脏细胞结构变化、细胞凋亡及相关基因或表面标志物变化。结果实验期间有23只小鼠怀孕,vplu蛋白和(或)抗vplu蛋白mAh干预组与对照组均未见胎鼠流产。各组产仔数量及鼠心脏重量进行组间方差分析,差异无显著性(P>0.05)。vplu蛋白和(或)抗VPIu蛋白mAl干预组光镜下均可见心肌细胞间隙增宽,胞质透明淡染,部分心肌细胞脂肪变性。电镜下vplu蛋白(或)和vplu蛋白mAh共同注射组、抗VPIu蛋白mAb注射组均可见线粒体聚集在增宽的心肌细胞间隙中,有空泡化,并TUNEL免疫荧光和免疫组织化学显示心肌细胞发生细胞凋亡。对照组胎鼠均未见异常。结论B19病毒vplu蛋白与胎鼠流产、心脏发育畸形无关,与胎鼠心脏水肿有关。
Objective To investigate whether vplu protein of B19 affects cardiac development and its mechanism. Methods The B19 virus vplu protein was injected into pregnant females at different gestational ages and fetal rat heart tissue samples were collected. The changes of cardiac cell structure, apoptosis and related genes or surfaces were observed by light microscopy, electron microscopy, immunohistochemistry, TUNEL and in situ hybridization Marker changes. Results Twenty-three mice were pregnant during the experiment. Fetal abortion was not observed in both the vplu and / or anti-vplu mAb intervention groups and the control group. There was no significant difference between groups (P> 0.05). Vplu protein and (or) anti-VPIu mAl intervention group light microscopy showed widened myocardial cell gap, the cytoplasm is light and transparent, some of the myocardial cell steatosis. Under the electron microscope, vplu protein or vplu protein mAh co-injection group, anti-VPIu protein mAb injection group can be seen mitochondria gathered in the widened myocardial interstitial space, vacuolization, and TUNEL immunofluorescence and immunohistochemistry showed that myocardial cells Apoptosis occurs. Fetal rats in the control group showed no abnormalities. Conclusion The B19 virus vplu protein has nothing to do with fetal rat miscarriage and heart malformation, and is related to heart edema in fetal rats.