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TiO2 film was prepared on soda-lime glass by sol-gel method. The water contact angle (θw) of the fresh TiO2 film is 0o. During storage in air, the surface of TiO2 film is gradually converted to the hydrophobic state. XPS and ITD results reveal that it is due to the adsorption of organic contaminants on TiO2 surface in air ambience. The lost hydrophilicity of TiO2 film can be regenerated by UV illumination.