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吲哚 -3 -乙酸和吲哚 -3 -丁酸在离子色谱仪Dionex 4 0 0 0i上使用荧光检测器进行检测。该测定采用 1 .0ml/min的 0 .4 5V/VCH3OH ,0 .0 1mol/lNa2 CO3的混合溶液作为淋洗液在DinoexOmniPacPAX -50 0柱分离。吲哚 -3 -乙酸和吲哚 -3 -丁酸的检测限分别为 0 .0 4 5μg/ml和 0 .1 67μg/ml,有良好的重现性和线性关系。对土壤样品进行测定 ,有较好的回收率。
Indole-3-acetic acid and indole-3-butyric acid were detected on an ion chromatograph Dionex 400i using a fluorescence detector. The assay was performed on a Dinoex OmniPac PAX-50 0 column using a mixed solution of 1.04 V / VCH3OH and 0.010 mol / l Na2C03 as eluant at 1.0 ml / min. The detection limits of indole-3-acetic acid and indole-3-butyric acid were 0. 04μg / ml and 01 67μg / ml, respectively, with good reproducibility and linearity. Soil samples were measured, a better recovery.