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使用低浓度H2O2和高浓度NaCl诱导稻瘟病菌的凋亡,检测了产生的凋亡特征。结果显示:诱导后稻瘟病菌具有典型的凋亡特征,如胞内活性氧累积、磷脂酰丝氨酸从细胞膜的内表面转移到外表面、染色体浓缩和DNA断裂。进一步研究发现:凋亡关键性酶类caspase的抑制剂Z-VAD-FMK能够显著影响稻瘟病菌的孢子萌发和附着胞形成过程。典型的稻瘟病菌孢子由3个细胞组成;在Z-VAD-FMK的作用下,2个以上细胞同时萌发产生芽管的比例由34.1%增加到66.0%,同时形成附着胞的源细胞也以顶端细胞为主转变为以基部细胞为主。这些结果说明凋亡参与了稻瘟病菌的孢子萌发和附着胞形成过程。
The apoptosis of Magnaporthe grisea was induced by using low concentration of H2O2 and high concentration of NaCl, and the characteristics of apoptosis were detected. The results showed that the induced M. grisea had typical apoptotic features, such as accumulation of intracellular reactive oxygen species, transfer of phosphatidylserine from the inner surface to the outer surface of the cell membrane, chromosome enrichment and DNA fragmentation. Further study found that: Z-VAD-FMK, a caspase inhibitor of apoptosis, could significantly affect the spore germination and attachment formation of Magnaporthe grisea. A typical strain of Magnaporthe grisea spores consisted of three cells. The proportion of germinating germinant cells from two or more cells increased from 34.1% to 66.0% under the action of Z-VAD-FMK, The apical cells mainly changed to basal cells. These results indicate that apoptosis is involved in spore germination and attachment formation of Magnaporthe grisea.