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以烟草品种K326为材料,比较水杨酸(SA)、甜菜碱(GB)、聚乙二醇(PEG 6000)和5-氨基乙酰丙酸(5-ALA)4种生理活性物质对烟草幼苗低温抗性生理指标的影响,对其改善烟草低温抗性的生理效果进行了评价。结果表明,与对照(清水)相比,4种生理活性物质可使遭遇低温胁迫的烟草幼苗维持较高水平的过氧化氢酶活性和叶绿素含量,可溶性糖和游离脯氨酸多量积累,丙二醛(MDA)含量和叶片电解质外渗率降低,表现出了增强烟草低温逆境抗性的潜力,且5-氨基乙酰丙酸、水杨酸和甜菜碱的生理效果略优于聚乙二醇,为烟草苗期低温耐性调节剂有效组分的初步筛选提供了理论依据。
Tobacco variety K326 was used to study the effects of four physiologically active substances, SA, GB, PEG 6000 and 5-ALA, on the effects of low temperature Resistant physiological indicators of its impact on improving the low temperature resistance of tobacco physiological effects were evaluated. The results showed that compared with the control (fresh water), four kinds of physiologically active substances could maintain high levels of catalase and chlorophyll content, soluble sugar and free proline accumulation in tobacco seedlings exposed to low temperature stress, Aldehyde (MDA) content and leaf electrolyte leakage rate decreased, showing the potential to enhance low temperature stress resistance of tobacco, and the physiological effects of 5-aminolevulinic acid, salicylic acid and betaine were slightly better than those of polyethylene glycol, This study provides a theoretical basis for the preliminary screening of effective components of tobacco seedling low temperature tolerance regulator.