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目的观察六经头痛片对大鼠离体血管平滑肌收缩活动及三叉神经降钙素基因相关肽(CGRP)表达的影响。方法制备大鼠离体主动脉血管环,采用麦氏浴槽法观察六经头痛片对血管平滑肌收缩活动的影响;培养三叉神经节组织,观察六经头痛片对三叉神经节CGRP表达的影响。结果六经头痛片可以减弱KCl及去甲肾上腺素(NE)引起的胸主动脉环收缩反应,对KCl收缩的半数抑制浓度(IC_(50))为0.97 mg/m L,对NE收缩的IC_(50)为0.55 mg/m L。而且,六经头痛片在1.4、0.14mg/m L时可明显减少离体培养24、48 h后三叉神经节CGRP阳性细胞的数量(P<0.05)。结论六经头痛片既可以对抗血管平滑肌的收缩,又可以降低三叉神经具有扩张血管作用的CGRP的量,说明六经头痛片具有稳定血管舒缩的作用。
Objective To observe the effects of Liujing Toutuo Pian on the contractile activity of isolated vascular smooth muscle and the expression of calcitonin gene related peptide (CGRP) in rats. Methods The isolated rat aorta rings were prepared and the effects of Liujingtou Tongpian Tablet on the contractile activity of vascular smooth muscle were observed by Maxillary bath. The effects of Liujing Toutuo Tablet on the expression of CGRP in the trigeminal ganglion were observed. Results Six-headache tablets could attenuate the contraction of thoracic aorta ring induced by KCl and NE, and the IC 50 of the KCl contraction was 0.97 mg / m L, (50) of 0.55 mg / m L. Moreover, the amount of CGRP-positive cells in trigeminal ganglion after six-head-headache tablets at 1.4 and 0.14mg / m L significantly decreased (P <0.05) 24 and 48 h after culture. Conclusion Six-headache tablets can not only inhibit the contraction of vascular smooth muscle, but also reduce the trigeminal nerve with dilation of blood vessels of CGRP amount, indicating that the six menstrual painkillers with a stable vasomotor effect.