论文部分内容阅读
目的观察姜黄素对泡状棘球蚴原头节生长的影响。方法将体外培养的泡状棘球蚴原头节分为6组:空白对照组,溶剂对照组和姜黄素20、40、60、80μmol/L组,观测原头节活性并计算存活率;姜黄素作用原头节24h后,应用半胱天冬氨酸蛋白酶-3(caspase-3)检测试剂盒检测caspase-3活性,应用原位末端脱氧核糖核苷酸转移酶标记技术(TUNEL法)试剂盒检测原头节细胞凋亡情况。结果培养9d后,空白对照组与溶剂对照组原头节活力无明显改变,各浓度姜黄素干预组原头节存活率与对照组相比差异均有统计学意义(F80μmol/L=4.986,F60μmol/L=4.826,F40μmol/L=4.364,F20μmol/L=3.288,P<0.05)。姜黄素对原头节的抑制作用呈时间、浓度依赖,80μmol/L姜黄素作用9d时原头节全部死亡。不同浓度姜黄素组作用24h原头节,caspase-3活性与对照组相比显著增高(F=446.937,P<0.05)。TUNEL法检测姜黄素干预原头节阳性率显著高于对照组(χ2=7.977,P<0.05)。结论姜黄素对体外培养的原头节有一定杀灭作用且具有时间和浓度依赖性。姜黄素对头节的杀灭作用可能与诱导头节细胞凋亡有关,其机制尚有待进一步研究。
Objective To observe the effect of curcumin on the growth of protoscoid Echinococcus granulosus. Methods The primary cultured heads of Echinococcus granulosus were divided into 6 groups: blank control group, solvent control group and curcumin 20, 40, 60 and 80μmol / L group. The activity of protoscoleces was observed and the survival rate was calculated. Caspase-3 activity was detected by caspase-3 assay kit after treatment with procambium for 24 h. The TUNEL method was used to detect the activity of caspase-3 Box detection of the original cell apoptosis. Results After cultured for 9 days, the viability of prorocement in blank control group and solvent control group had no significant change. The survival rate of proroca in each concentration of curcumin intervention group was significantly different from that in control group (F80μmol / L = 4.986, F60μmol /L=4.826,F40μmol/L=4.364,F20μmol/L=3.288,P<0.05). Curcumin inhibited the protoscoleces in a time-and concentration-dependent manner. All of the protoscoleces died when treated with 80 μmol / L curcumin for 9 days. The activity of caspase-3 in different concentrations of curcumin group was significantly higher than that in the control group (F = 446.937, P <0.05). The positive rate of TUNEL assay of curcumin in intervention of the prothorax was significantly higher than that of the control group (χ2 = 7.977, P <0.05). Conclusion Curcumin has a certain killing effect on protoplasm cultured in vitro and in a time and concentration-dependent manner. The killing effect of curcumin on the head section may be related to the induction of apoptosis of the head section, the mechanism remains to be further studied.