以小麦印度腥黑穗病菌和黑麦草腥黑粉菌为研究对象,采用分子克隆技术,分别构建了该两种真菌分子检测的标准分子。前者包括线粒体2297 bp的DNA序列以及rDNA710 bp的ITS序列,后者包括线粒体2.3kb的DNA序列以及rDNA710 bp的ITS序列。分别对该两个标准分子进行了性能评估试验,测试结果显示所构建的标准分子具有良好的特异性、均匀性和稳定性,能够满足小麦印度腥黑穗病菌和黑麦草腥黑粉菌分子检测需求。 Triticum aestivum and Triticum aestivum were used as research objects, and molecular cloning techniques were used to construct the standard molecules for the detection of the two fungal molecules respectively. The former includes the 2297 bp DNA sequence of mitochondria and the ITS sequence of 710 bp rDNA, which contains the 2.3 kb mitochondrial DNA sequence and the rDNA 710 bp ITS sequence. The performance evaluation of the two standard molecules were carried out respectively. The test results show that the standard molecules have good specificity, uniformity and stability, which can meet the molecular detection of Triticum aestivum and Triticum aestivum demand.