论文部分内容阅读
目的：分析血链球菌染色体ＤＮＡ片段序列，探讨血链球菌遗传生物学特性。方法：采用ＴＡ克隆技术将此片段转化克隆并进行碱基序列测定，通过ＧｅｎＢａｎｋ进行同源分析。结果：血链球菌ＡＴＣＣ１０５５６ＤＮＡ片段含有与固氮菌高度同源的ｎｉｆＳ、ｎｉｆＵ基因，同源分值为１１４点（ｂｉｔｓ）。结论：国内外首次报道血链球菌ＤＮＡ片段含有ｎｉｆＳ、ｎｉｆＵ部分基因。
Objective: To analyze the sequence of chromosomal DNA of Streptococcus sanguis and explore the genetic biological characteristics of Streptococcus sanguis. Methods: The fragment was cloned by TA cloning technique and sequenced. The homology was analyzed by GenBank. Results: The S.niger ssp. ATCC10556 DNA fragment contained highly homologous nifS and nifU genes to Azotobacter, with a homology score of 114 bits. Conclusion: For the first time at home and abroad reported that S streptococcus DNA fragment contains nifS, nifU part of the gene.