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目的:检测小鼠口腔癌淋巴道转移模型癌变过程不同时期的骨髓播散肿瘤细胞(disseminated tumor cell,DTC),探讨小鼠口腔癌变过程与骨髓DTC的关系。方法:病理检查明确诊断为正常舌黏膜、舌单纯性增生的小鼠各10只,舌轻中度异常增生、舌重度异常增生、舌鳞癌的小鼠各20只。采用Ficoll密度梯度离心法提取小鼠股骨骨髓里单个核细胞并制成细胞涂片,免疫细胞化学(Immunocytochemistry,ICC)检测骨髓DTC,比较其数目差异。结果:舌重度异常增生8只、舌鳞癌组13只发现DTC,阳性率分别为40%(8/20)和65%(13/20),每100个单核细胞中DTC数分别为3.03±0.75个和5.20±0.74个,差异均有统计学意义(P<0.05)。其余各组均未发现DTC(0/10)。结论:小鼠舌黏膜恶变过程中,舌重度异常增生时已出现DTC,并随病变程度加重,骨髓DTC的发生率及其细胞数量增加。
OBJECTIVE: To detect disseminated tumor cells (DTCs) in different stages of carcinogenesis in mouse oral lymphatic metastasis model and to investigate the relationship between oral carcinogenesis and bone marrow DTC in mice. Methods: 10 pathologically confirmed normal tongue mucosa, 10 hyperplastic mice with tongue hyperplasia, mild to moderate hyperplasia of tongue, severe tongue hyperplasia, and 20 from tongue squamous cell carcinoma. Ficoll density gradient centrifugation was used to extract the bone marrow mononuclear cells from femurs and made into smears. Immunocytochemistry (ICC) was used to detect bone marrow DTCs, and their numbers were compared. RESULTS: There were 8 severe tongue dysplasia and 13 DTC in tongue squamous cell carcinoma, the positive rates were 40% (8/20) and 65% (13/20), respectively. The number of DTCs per 100 monocytes was 3.03 ± 0.75 and 5.20 ± 0.74 respectively, the differences were statistically significant (P <0.05). No other group found DTC (0/10). CONCLUSION: During the malignant process of tongue mucosa in mice, DTC has appeared in severe tongue hyperplasia, and with the increase of lesion degree, the incidence of DTC and the number of cells in bone marrow increased.