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目的:探讨整合素αvβ6、MMP-9在胃癌中表达及其与胃癌病理生物学特征的关系。方法:采用EnVision免疫组化方法检测94例胃癌及癌旁胃黏膜组织中整合素αvβ6、MMP-9的表达情况,并分析两者表达与胃癌病理生物学特征的关系。结果:胃癌组织中整合素αvβ6和MMP-9阳性表达率明显高于癌旁胃黏膜组织(P<0.01)。整合素αvβ6在胃癌组织中的表达与Lauren分型、组织分化程度、是否有淋巴结转移(N分期)以及TNM分期密切相关(P<0.01),而MMP-9在胃癌组织中的表达与Lauren分型、组织分化程度、是否有淋巴结转移(N分期)、浸润深度以及TNM分期密切相关(P<0.01)。在胃癌组织中整合素αvβ6和MMP-9的表达呈正相关(r=0.672,P<0.01)。整合素αvβ6和MMP-9表达阴性患者的5年生存率明显优于整合素αvβ6和MMP-9表达阳性的患者(P<0.01)。Cox回归多因素分析表明Lauren分型、是否有淋巴结转移(N分期)、整合素αvβ6和MMP-9高表达是影响患者5年生存率的独立预后因素(P<0.05)。结论:整合素αvβ6和MMP-9的高表达与胃癌的浸润、转移密切相关;两者表达可作为判断胃癌患者预后的参考指标及治疗的靶向目标。
Objective: To investigate the expression of integrin αvβ6 and MMP-9 in gastric carcinoma and its relationship with the pathobiological characteristics of gastric carcinoma. Methods: EnVision immunohistochemical method was used to detect the expression of integrin αvβ6 and MMP-9 in 94 cases of gastric cancer and adjacent gastric mucosa. The relationship between them and the pathological and biological characteristics of gastric cancer was analyzed. Results: The positive rates of integrin αvβ6 and MMP-9 in gastric cancer tissues were significantly higher than those in adjacent gastric mucosa tissues (P <0.01). The expression of integrin αvβ6 in gastric carcinoma was closely related to Lauren’s classification, histological differentiation, lymph node metastasis (N stage) and TNM stage (P <0.01), while the expression of MMP-9 in gastric carcinoma was correlated with Lauren’s score Type, histological differentiation, lymph node metastasis (N stage), depth of invasion and TNM stage (P <0.01). The expression of integrin αvβ6 and MMP-9 in gastric cancer tissues was positively correlated (r = 0.672, P <0.01). The 5-year survival rate of patients with negative expression of integrin αvβ6 and MMP-9 was significantly better than those with positive expression of integrin αvβ6 and MMP-9 (P <0.01). Cox regression multivariate analysis showed that Lauren classification, whether lymph node metastasis (N stage), integrin αvβ6 and MMP-9 overexpression were independent prognostic factors (P <0.05). Conclusion: The overexpression of integrin αvβ6 and MMP-9 is closely related to the infiltration and metastasis of gastric cancer. The expression of both integrin αvβ6 and MMP-9 may serve as a reference index for prognosis and target of treatment.