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目的 探讨胚胎大鼠神经干细胞体外培养分化的子代细胞电生理特性。方法 传代细胞单层贴壁培养 ,细胞内电流钳记录静息膜电位、动作电位、膜阻抗等电生理指标。结果 神经干细胞在现有培养条件下可初步分化为神经元和胶质细胞 ,并表现出不同的电生理特性。结论 神经干细胞表现出多向分化潜能 ,但现有培养条件尚不能使子代细胞电生理功能完全成熟
Objective To investigate the electrophysiological characteristics of embryonic rat neural stem cells cultured in vitro and in vitro. Methods Adherent cells cultured in monolayer were cultured with intracellular current clamp to record the resting membrane potential, action potential, membrane impedance and other electrophysiological indexes. Results Neural stem cells differentiated into neurons and glial cells under the existing culture conditions and showed different electrophysiological properties. Conclusion Neural stem cells show the potential of multidrug differentiation, but the current culture conditions are not yet enough to fully mature the electrophysiological function of progeny cells