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在接种S_(180)肉瘤细胞0、7、14、21和28天,分别从该瘤小鼠脾脏抽提RNA,将这些RNA制剂与S_(180)瘤细胞一起注入实验鼠皮下,比较它们的体内抗肿瘤作用,发现7～21天抽提的RNA有明显抗肿瘤作用,并以14天者为最强。通过体外~3H-UR参入带S_(180)肉瘤小鼠脾细胞的测定,观察RNA的合成,显示小鼠患瘤第4天其脾细胞RNA合成速率即加速,14天增至三倍。实验结果表明:RNA的抗肿瘤作用有赖于RNA的合成性状,即脾细胞RNA合成得愈快、愈多,此时抽提的RNA抗肿瘤作用则愈强。
RNA was extracted from the tumor mouse spleen at 0, 7, 14, 21, and 28 days after inoculation with S_(180) sarcoma cells. These RNA preparations were injected together with S_(180) tumor cells under the skin of rats to compare their In vivo anti-tumor effect, it was found that RNA extracted from 7 to 21 days had significant anti-tumor effect and was the strongest in 14 days. The in vitro 3H-UR incorporation of splenocytes from S_(180) sarcoma mice was used to observe the synthesis of RNA. It was shown that the RNA synthesis rate of spleen cells of mice at the 4th day of tumor growth was accelerated and increased to three times from 14 days. The experimental results show that the anti-tumor effect of RNA depends on the synthesis characteristics of RNA, that is, the faster the RNA synthesis of spleen cells, the more the anti-tumor effect of extracted RNA is stronger.