[目的 ]研究粘附分子 TNF- α、ICAM- 1与脑型疟 (cerebral malaria,CM)的关系 ,并通过体内注射外源性 TNF- α来观察 ICAM- 1的表达情况及其对 CM发生的影响。 [方法 ]通过建立 CM小鼠模型 ,酶联免疫吸附实验 (EL ISA)检测感染小鼠血清 TNF-α浓度。免疫组织化学 SP法检测感染小鼠脑微血管的 ICAM- 1表达 ,结果用真彩色图象分析仪半定量分析。 [结果 ]发生 CM小鼠的血清 TNF- α明显高于其它小鼠 ,只有发生 CM的小鼠脑微血管有 ICAM- 1表达 ,体内注射 r TNF-α能促进 CM的发生 ,并显著增加脑微血管的 ICAM- 1的表达。 [结论 ]大量的 TNF- α在 CM的发病中可能有直接致病作用 ,但主要可能通过调节脑微血管的 ICAM- 1表达发挥作用 [Objective] To study the relationship between the expression of TNF-α, ICAM-1 and cerebral malaria (CM) and to observe the expression of ICAM-1 and its effect on CM by injecting exogenous TNF- Impact. [Method] The serum concentration of TNF-αin infected mice was detected by ELISA and ELISA. Immunohistochemical SP method was used to detect the expression of ICAM-1 in the cerebral microvessels of infected mice. The results were semi-quantitatively analyzed with true color image analyzer. [Results] The serum level of TNF-α in CM mice was significantly higher than that of the other mice, only ICAM-1 was expressed in the brain microvessels of CM mice. In vivo r TNF-α injection could promote the development of CM and significantly increase the cerebral microvascular Of ICAM-1 expression. [Conclusion] A large number of TNF-α may play a direct pathogenic role in the pathogenesis of CM, but may play a role mainly through the regulation of ICAM-1 expression in brain microvessels