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目的:建立大鼠心肌梗死后蛋白表达变化谱,以进一步了解心肌梗死后心肌细胞重塑产生机制。方法:通过结扎大鼠冠状动脉左前降支建立急性心肌梗死模型,利用蛋白质双向凝胶电泳技术(two-dimensionalgelelectrophoresis,2-DE)分离心肌总蛋白,采用PDQuest7.3.1软件比较分析,获得差异表达蛋白总体变化趋势。进一步通过蛋白免疫印记技术(Western-blotting)检测碱性成纤维生长因子(Basefibroblastgrowthfactor,bFGF)在心肌梗死后的表达变化。结果:成功建立了大鼠急性心肌梗死模型;2-DE结果表明:以假结扎组为对照,梗死3天组有27个蛋白显著上调,18个蛋白显著下调,7个蛋白表达明显差异(ratio>5)。进一步研究发现梗死区心肌组织bFGF表达明显升高。结论:心肌梗死后蛋白表达变化趋势的探讨为心室重塑机制研究提供线索。
Objective: To establish a spectrum of protein expression changes after myocardial infarction in rats to further understand the mechanism of cardiomyocyte remodeling after myocardial infarction. Methods: The model of acute myocardial infarction was established by ligation of the left anterior descending coronary artery in rats. The total protein of myocardium was separated by two-dimensional gel electrophoresis (2-DE). PDQuest7.3.1 software was used to compare the expression of differentially expressed protein The overall trend of change. The expression of basic fibroblast growth factor (bFGF) after myocardial infarction was detected by Western-blotting. Results: The model of acute myocardial infarction in rats was successfully established. The results of 2-DE showed that in the fake ligation group, 27 proteins were significantly up-regulated, 18 proteins were significantly down-regulated and the expression of 7 proteins was significantly decreased > 5). Further study found that myocardial infarction area bFGF expression was significantly increased. Conclusion: The study of the changes of protein expression after myocardial infarction provides clues for the study of ventricular remodeling mechanism.