p38丝裂原活化蛋白激酶抑制剂对创伤失血性休克致急性肺损伤大鼠的影响

来源 :武汉大学学报(医学版) | 被引量 : 0次 | 上传用户:kuo1314
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的:探讨p38丝裂原活化蛋白激酶抑制剂SB203580对创伤失血性休克致急性肺损伤大鼠的影响。方法:30只SPF级健康雄性SD大鼠随机分为3组(n=10):假手术组(S组)、急性肺损伤模型组(M组)和急性肺损伤模型组+SB203580组(SB组)。M组和SB组建立急性肺损伤模型,SB组造模前30 min静脉注射抑制剂SB203580(2mg/kg)。股动脉穿刺置管监测平均动脉压(MAP)和心率(HR);创伤后6h采集动脉血样进行血气分析;采用ELISA法测定血清TNF-α、IL-6和IL-1β的水平;收集肺泡灌洗液(BALF)行中性粒细胞细胞(PMN)计数;比色法测定肺组织髓过氧化物酶(MPO)活性;光镜和电镜下观察肺组织病理学改变,采用Western blot法检测p38丝裂原活化蛋白激酶(p38MAPK)的表达。结果:创伤失血性休克后2h和4h时间点,大鼠心率和平均动脉血压显著下降,静脉注射SB203580后大鼠心率和平均动脉血压相对平稳。与S组比较,M组大鼠pH和PaO2降低,PaCO2、BALF中中性粒细胞细胞计数、MPO活性和血清TNF-α、IL-6及IL-1β的水平升高,肺组织p38MAPK的表达上调(P<0.05);与M组比较,SB组大鼠pH和PaO2升高,PaCO2、BALF中中性粒细胞细胞计数、MPO活性和血清TNF-α、IL-6及IL-1β的水平降低,肺组织p38MAPK的表达下调(P<0.05)。结论:p38MAPK抑制剂SB203580可减少肺组织中的p38MAPK的表达,降低血清中的TNF-α、IL-6和IL-1β的水平,从而减轻创伤失血性休克导致的急性肺损伤。 Objective: To investigate the effect of p38 mitogen-activated protein kinase inhibitor SB203580 on acute lung injury induced by traumatic hemorrhagic shock in rats. Methods: Thirty healthy SPF male SD rats were randomly divided into three groups (n = 10): sham operation group (S group), acute lung injury model group (M group) and acute lung injury model group + SB203580 group group). Acute lung injury model was established in M ​​group and SB group, and SB203580 (2 mg / kg) was injected intravenously 30 minutes before model group in SB group. The arterial blood pressure (MAP) and heart rate (HR) were monitored by femoral artery catheterization. Arterial blood samples were collected 6h after trauma for blood gas analysis. Serum levels of TNF-α, IL-6 and IL-1β were measured by ELISA. (PMN) were counted in the lotion (BALF); the activity of myeloperoxidase (MPO) in lung tissue was measured by colorimetric method; the pathological changes of lung tissue were observed under light microscope and electron microscope; Mitogen-activated protein kinase (p38MAPK) expression. Results: After 2h and 4h of traumatic hemorrhagic shock, the heart rate and mean arterial blood pressure of rats decreased significantly. The heart rate and mean arterial pressure of rats after SB203580 intravenous injection were relatively stable. Compared with group S, the pH and PaO2 of group M were decreased, the number of neutrophil cells, the activity of MPO and the level of TNF-α, IL-6 and IL-1β were increased in PaCO2 and BALF, and the expression of p38MAPK (P <0.05). Compared with M group, the pH and PaO2 of SB group increased, the level of PaCO2 and neutrophil count, MPO activity and the level of serum TNF-α, IL-6 and IL-1β in BALF Decreased, the expression of p38MAPK in lung decreased (P <0.05). CONCLUSIONS: p38MAPK inhibitor SB203580 can reduce the expression of p38MAPK in lung tissue and decrease the levels of TNF-α, IL-6 and IL-1β in serum and thus reduce the acute lung injury induced by traumatic hemorrhagic shock.
其他文献
目的:探讨腹膜癌患者接受肿瘤细胞减灭(cytoreductive surgery,CRS)联合腹腔热灌注化疗(hyperthermic intraperitoneal chemotherapy,HIPEC)术后血肌红蛋白(myoglobin,Mb)水
近年来,随着我国社会经济的高速发展和科学技术的不断进步,网络信息产业由此获得了显著的发展,信息增长速度不断加快,相应的促使现代社会逐渐进入大数据时代。当下我国城镇化
以阜新市"中绥12号"杨树林分为研究对象,对1年生杨树进行鸡粪与尿素不同配比施肥试验,以分析施肥对根际土壤养分、土壤微生物数量等指标的影响。结果表明:"J30N70"的施肥处理
回 回 产卜爹仇贱回——回 日E回。”。回祖 一回“。回干 肉果幻中 N_。NH lP7-ewwe--一”$ MN。W;- __._——————》 砧叫]们羽 制作:陈恬’#陈川个美食 Back to yield
甘孜-理塘断裂带上,四川德格县错阿乡地区的一系列逆冲推覆岩席及其从属的飞来峰群可归并为三大逆冲推覆体系。本文描述了推覆断裂及推覆岩席的典型特征,以及滑动断层面呈犁式的
目的:探讨寰枢椎半脱位患儿接受脊柱旋转定点复位手法治疗的效果。方法:收集80例寰枢椎半脱位患儿的临床资料,对其采用脊柱旋转定点的方法进行复位治疗,并结合中药热敷以及消
本文在调研国内外旅游气象服务现状、旅游管理部门以及公众对气象服务需求的基础上,凝练和总结了当前我国旅游气象服务中存在的不足和问题,并对下一步的发展方向进行了思考,
在实体经济中房地产与诸多相关产业存在密切产业链联动机制。本文采用马尔可夫区制转换向量自回归模型(MS-VAR)来分析房地产业与相关产业(钢材、水泥、家具、家电)之间的联动
搭材作:原名扎材作,所谓“扎”即捆、绑、缠、束之意,所谓“材”泛指古建筑的定形构件,扎材的作业是用杉木杆及粗细麻绳为工具,以各种捆绑的技巧及吊、拉、顶、垫的方法。对