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目的 探讨丹参(Radix saliviae miltiorrizae,RSM)在肝纤维化过程中的作用。方法 采用大鼠腹腔注射硫代乙酰胺(TAA),复制肝纤维化模型。分别给不同组大鼠腹腔注射TAA和皮下注射丹参,13周后观察谷丙氨酸转氨酶(ALT)、乳酸脱氢酶(LDH)、血浆内毒素LPs含量、血浆和肝组织NO含量、HE和V·G染色观察各组肝组织形态改变。结果 TAA组:ALT、LDH、LPS羟脯氨酸、血浆和肝组织NO含量均高于正常对照组(P<0.05),丹参组上述指标均低于TAA组,组织学观察丹参组肝损伤和纤维化程度较TAA组轻。结论 丹参在TAA诱导的肝纤维化过程中有保护作用。
Objective To investigate the role of Radix saliviae miltiorrizae (RSM) in liver fibrosis. METHODS Rats were intraperitoneally injected with thioacetamide (TAA) to replicate liver fibrosis model. TAA and subcutaneous injection of Salvia miltiorrhiza were given intraperitoneally to rats in different groups. After 13 weeks, the contents of alanine aminotransferase (ALT), lactate dehydrogenase (LDH), plasma endotoxin LPs, plasma and liver NO, and HE were measured. V.G staining was used to observe the morphological changes of liver tissue in each group. Results The contents of NO in ALT, LDH, LPS, hydroxyproline, plasma and liver tissue in TAA group were higher than those in normal control group (P<0.05). The above indexes in Salvia miltiorrhiza group were lower than those in TAA group. Histological observation of liver injury in Salvia miltiorrhiza group was performed. The degree of fibrosis was lighter than that of the TAA group. Conclusion Salvia Miltiorrhiza has a protective effect on TAA-induced hepatic fibrosis.