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用Omura及Johannesson等报道的方法测定大白鼠肝微粒体悬液(S—9)中P450含量。实验发现,对照组大白鼠肝微粒体中P450含量为25.6～33.8nM/g肝,iP苯巴比妥钠50mg/kg/天×3,P-450含量可升高2.6～3.2倍,四氯化碳则使之减少72%。冬凌草及其有效成份冬凌草甲素、乙素可使大白鼠肝微粒体P450含量降低40～50%,冬凌草甲素并可抗苯巴妥及三氯联苯之诱导作用。乙双吗啉,乙亚胺及丙亚胺等双内酰亚胺化合物亦可降低P450含量;常用抗肿瘤药5-氟尿嘧啶及环磷酸胺亦可使大白鼠肝微粒体P450含量下降。有关其作用机制及意义进行了讨论。
The content of P450 in rat liver microsomal suspension (S-9) was determined by methods reported by Omura and Johannesson et al. The experiment found that the control group rats liver microsomes P580 content of 25.6 ~ 33.8nM / g liver, iP phenobarbital sodium 50mg/kg/day × 3, P-450 content can be increased 2.6 ~ 3.2 times, tetrachlorin Carbon is reduced by 72%. Oridonin and its effective components Rubescensine A, B can reduce the liver microsomal P450 content of rats by 40 to 50%, Oridonin can resist the induction of phenobarbital and trichlorobiphenyl. Bis-morpholine, bis-imine and imine imine can also reduce the content of P450. Commonly used anti-tumor drugs 5-fluorouracil and cyclophosphamide can also reduce the content of P450 in rat liver microsomes. The discussion of its mechanism and significance.