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目的建立高效液相色谱-串联质谱联用技术检测茶叶中赭曲霉毒素A(OTA)的方法。方法用甲醇-2%Na HCO3溶液(60:40,V:V)提取样品中的赭曲霉毒素A,采用免疫亲和柱对茶叶中的赭曲霉毒素A净化,使用甲醇-5 mmol/L乙酸铵(含0.1%乙酸)为流动相,检测赭曲霉毒素A,采用正离子模式。结果本方法中赭曲霉毒素A在0.5~10 ng/m L质量浓度范围内具有良好的线性关系(r=0.996),回收率在83.6%~92.5%之间,相对标准偏差在6.5%~9.1%之间,检出限为0.1μg/kg。结论该方法准确性好、灵敏度高,适用于茶叶样品的检测。
Objective To establish a method for the determination of ochratoxin A (OTA) in tea using high performance liquid chromatography-tandem mass spectrometry. Methods The ochratoxin A in the sample was extracted with methanol-2% Na HCO3 solution (60:40, V: V) and the ochratoxin A in tea was purified by immunoaffinity column using methanol-5 mmol / L acetic acid Ammonium (0.1% acetic acid) was used as the mobile phase to detect ochratoxin A in positive ion mode. Results The results showed that ochratoxin A had a good linearity (r = 0.996) in the range of 0.5 ~ 10 ng / mL and the recoveries ranged from 83.6% to 92.5% with relative standard deviations of 6.5% ~ 9.1 %, The detection limit of 0.1μg / kg. Conclusion The method is accurate, sensitive and suitable for the detection of tea samples.