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目的 :探索可靠反映血吸虫病肝纤维化的定量方法。方法 :将 86例晚期血吸虫病患者的肝组织标本 ,分别做HE、胶原纤维及弹力纤维染色。光镜下对胶原纤维进行半定量计分 ;用计算机图像分析系统测定全胶原表面密度 (SDTC)及全弹力表面密度 (SDTE) ;用比色法测定肝组织中的胶原含量。结果 :肝组织中SDTC与SDTE呈正相关 (r=0 .76 ,P <0 .0 0 1) ;SDTC与比色法测得胶原含量呈显著性相关 (r =0 .78,P <0 .0 0 1)。以SDTC为标准分组 ,比较SSS计分及比色法测定的胶原含量 ,结果组间差异均有显著性 (P <0 .0 1) ;SDTC与SSS及其各变量的多元逐步回归分析表明对SDTC起主要影响的因素是SSS。结论 :计分法、图像分析法及比色法均可用于血吸虫病肝纤维化的定量分析。比色法由于间便易操作 ,特异性强 ,重度性好 ,认为是肝纤维化定量检测的较好方法
Objective: To explore a reliable quantitative method for detecting liver fibrosis in schistosomiasis. Methods: Tissue specimens from 86 patients with advanced schistosomiasis were stained with HE, collagen and elastic fibers respectively. The collagen fibers were scored semi-quantitatively under light microscope. The total surface area (SDTC) and total surface area (SDTE) of collagen were measured by computer image analysis system. The content of collagen in liver tissue was determined by colorimetry. Results: There was a positive correlation between SDTC and SDTE in liver tissue (r = 0.76, P <0.01). There was a significant correlation between SDTC and colorimetry (r = 0.78, P <0. 0 0 1). SDTC as a standard group, compared with the SSS score and colorimetric determination of collagen content, the results were significant differences between groups (P <0. 01); SDTC and SSS and its multiple variables stepwise regression analysis showed that The main factor that influences SDTC is SSS. Conclusion: The scoring method, image analysis method and colorimetric method can be used for the quantitative analysis of liver fibrosis in schistosomiasis. Colorimetry is easy to operate because of the inter-specific, strong specificity, is considered a good method for the quantitative detection of liver fibrosis