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目的通过对硬化剂体外硬化处理后的肾囊肿进行病理学研究,以便为配合后腹腔镜肾囊肿手术时选择合适的硬化剂及其作用时间。方法分别选取500g/L葡萄糖溶液、200g/L硫酸铝钾溶液、无水乙醇、10%甲醛溶液(体积分数)、石碳酸溶液作为硬化剂。将硬化剂作用于囊肿壁内腔面,处理时间为30s及1、2、5、10min。处理后组织HE染色,观察囊肿壁变性坏死程度。结果500g/L葡萄糖、200g/L硫酸铝钾处理10min后仅有部分上皮细胞变性坏死;无水乙醇处理10min后有部分上皮细胞未发生变性坏死;10%(体积分数)甲醛溶液处理10min后所有上皮细胞均变性坏死;石碳酸溶液处理2min后所有上皮细胞变性坏死,且深达囊肿壁外层。结论选择石碳酸作为硬化剂配合后腹腔镜手术治疗肾囊肿,效果优于其他硬化剂。
Objective To study the pathological study of renal cysts after hardening sclerotherapy in vitro so as to select the suitable sclerotherapy for the operation of laparoscopic renal cyst surgery. Methods 500g / L glucose solution, 200g / L potassium aluminum sulfate solution, absolute ethanol, 10% formaldehyde solution (volume fraction) and carbolic acid solution were selected as hardeners. The hardener acting on the cyst wall surface, the treatment time is 30s and 1,2,5,10 min. After treatment, the tissue was stained with HE to observe the degree of degeneration and necrosis of the cyst wall. Results Only 500g / L glucose and 200g / L aluminum potassium sulfate only partially denatured and necrotic epithelial cells. After 10min of ethanol treatment, some epithelial cells did not degenerate and necrosis. After 10min of 10% formaldehyde solution treatment, Epithelial cells were degenerated and necrotic; all epithelial cells were degenerated and necrotic after 2min treatment with carbolic acid solution, and reached the outer layer of cyst wall. Conclusion The use of carbolic acid as sclerotherapy combined with laparoscopic surgery for renal cysts is superior to other sclerosing agents.