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目的:观察赛米司酮终止猕猴早孕的效果及对阴道出血量的影响,为新药研发提供实验依据。方法:妊娠猕猴随机分为7组,其中受试药赛米司酮和阳性对照药米非司酮均分为低、中、高剂量3个组(5 mg/kg.d、10 mg/kg.d和15mg/kg.d);另设一赋型剂组(0.5%羧甲基纤维素钠),每组动物各5只。于月经周期第42～48天,空腹状态下分别给予不同剂量的赛米司酮或米非司酮,1次/日,连续3天,第4天再加用米索前列醇200μg,观察用药后的不良反应,并于第15天经B超判断孕猴流产及胚胎发育状况。结果:赛米司酮高剂量组完全流产率为60.00%(3/5),中剂量组为20.00%(1/5),而低剂量组未见流产;阳性对照组米非司酮在相应剂量下完全流产率分别为80.00%(4/5)、40.00%(2/5)和20.00%(1/5)。赛米司酮组和米非司酮组总的流产率分别为26.67%(4/15)和46.67%(7/15),两组间的差异无统计学意义(P>0.05)。赛米司酮组阴道持续出血时间明显短于米非司酮组,出血量明显少于米非司酮组(P<0.05)。结论:赛米司酮能有效终止猕猴早孕,且能明显地缩短出血时间和减少出血量。
OBJECTIVE: To observe the effects of cypristine on termination of macaque pregnancy and vaginal bleeding, and provide experimental evidence for the development of new drugs. Methods: The pregnant cynomolgus monkeys were randomly divided into 7 groups. Among them, the test drug cyphenothione and the positive control mifepristone were divided into 3 groups (5 mg / kg.d, 10 mg / kg) .d and 15mg / kg.d); another set of excipients (0.5% sodium carboxymethyl cellulose), each group of five animals. In the menstrual cycle 42 to 48 days, were given different doses of cyproheptadine or mifepristone fasting state, 1 times / day for 3 days, 4 days plus misoprostol 200μg, observed medication After adverse reactions, and on the 15th day by B ultrasound to determine the pregnancy monkey abortion and embryonic development status. Results: The total abortion rate was 60.00% (3/5) in the high dose group and 20.00% (1/5) in the middle dose group, and no abortion was found in the low dose group. In the positive control group, mifepristone The rates of complete abortion were 80.00% (4/5), 40.00% (2/5) and 20.00% (1/5), respectively. The total miscarriage rate was 26.67% (4/15) and 46.67% (7/15) respectively in the patients treated with cyprodone and mifepristone. There was no significant difference between the two groups (P> 0.05). The duration of vaginal bleeding in the cytopril group was significantly shorter than that in the mifepristone group, with less bleeding than in the mifepristone group (P <0.05). Conclusion: Secretize ketamine can effectively terminate the first trimester of macaque, and can significantly shorten the bleeding time and reduce the amount of bleeding.