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目的研究巴戟天对手机辐射致雄性成年SD(Sprague-Dawley)大鼠下丘脑-垂体-性腺轴损伤的作用。方法 50只雄性成年SD大鼠随机平均分为5组:空白组(不辐射,不给药)、模型组(辐射,不给药)、双蒸水对照组(辐射,灌胃双蒸水)、实验A组(辐射,灌胃水提物)和实验B组(辐射,灌胃醇提物)。用手机辐射,频率为900 m Hz,比吸收率为0.76 W·kg~(-1)。持续辐射2周后,实验A、B组分别给予巴戟天水提物、醇提物20 g·kg~(-1)·d~(-1)灌胃,持续2周。计算附睾和睾丸指数;用酶联免疫吸附法检测血清促性腺激素释放激素(GnRH)、卵泡刺激素(FSH)、黄体生成素(LH)和血清睾酮(T)水平;用实时荧光PCR法检测睾丸组织中黄体生成素受体(LHR)和卵泡刺激素受体(FSHR)的mRNA的相对表达;计量精子相对计数、畸形率和活动率。结果睾丸指数:双蒸水对照组和实验A、B组分别为0.84±0.11,0.70±0.16,0.82±0.13;附睾指数:双蒸水对照组和实验A、B组分别为0.26±0.08,0.24±0.02,0.27±0.05;与双蒸水对照组相比,实验A、B组的附睾指数和睾丸指数差异无统计学意义(P>0.05)。FSH水平(U·L~(-1)):空白组、模型组、双蒸水对照组和实验A、B组分别为0.24±0.04,0.44±0.01,0.40±0.00,0.25±0.04,0.30±0.05;GnRH、LH和T水平(ng·L~(-1)):空白组分别为1.90±0.11,4.30±0.27,4.44±0.11;模型组分别为2.33±0.09,7.99±0.96,2.09±0.40;双蒸水对照组分别为2.33±0.11,8.04±0.36,2.00±0.29;实验A组分别为1.91±0.12,4.80±0.70,4.07±0.16;实验B组分别为1.99±0.09,5.00±0.29,3.89±0.26,组间比较差异有统计学意义(均P<0.05)。LHR mRNA在空白组、模型组、双蒸水对照组和实验A、B组的相对表达水平分别为1.00±0.00,0.67±0.03,0.66±0.05,0.91±0.04,0.90±0.07;FSHR mRNA在空白组、模型组、双蒸水对照组和实验A、B组的相对表达水平分别为1.00±0.00,0.51±0.05,0.50±0.07,0.81±0.06,0.80±0.08,组间比较差异有统计学意义(均P<0.05)。精子相对计数(×106):在空白组、模型组、双蒸水对照组和实验A、B组分别为168.03±11.25,79.17±8.62,80.06±10.13,143.39±6.35,140.25±9.41;精子畸形率:在空白组、模型组、双蒸水对照组和实验A、B组分别为6.03±072,20.57±1.05,21.07±1.04,6.20±0.88,6.99±1.02;精子活动率在空白组、模型组、双蒸水对照组和实验A、B组分别为87.13±1.63,45.98±3.02,48.09±2.97,71.13±4.02,69.08±1.12。与空白组比较,模型组精子相对计数、活动率降低和畸形率差异有统计学意义(均P<0.05);与双蒸水对照组比较,实验A、B组精子相对计数、活动率增高和畸形率差异有统计学意义(均P<0.05)。结论巴戟天对微波辐射致雄性成年SD大鼠下丘脑-垂体-性腺轴损伤有修复作用。
Objective To study the effects of Morinda officinalis on the hypothalamic-pituitary-gonad axis injury in male adult Sprague-Dawley rats induced by cellphone radiation. Methods Fifty adult male Sprague-Dawley rats were randomly divided into five groups: blank group (no radiation, no administration), model group (radiation, no administration), double distilled water control group , Experimental group A (radiation, gastric perfusion water) and experimental group B (radiation, gastric perfusion alcohol extract). The cell phone radiates at a frequency of 900 m Hz with a specific absorption rate of 0.76 W · kg -1. Two weeks after the continuous radiation, the experimental group A and group B were given Morinda officinalis water extract, ethanol extract 20 g · kg -1 d -1 for 2 weeks. The epididymis and testis index were calculated. Serum levels of gonadotropin-releasing hormone (GnRH), follicle stimulating hormone (FSH), luteinizing hormone (LH) and serum testosterone (T) were measured by enzyme-linked immunosorbent assay Relative expression of mRNA of luteinizing hormone receptor (LHR) and follicle stimulating hormone receptor (FSHR) in testis tissue; counting sperm relative counting, deformity rate and activity rate. Results Testis index: double distilled water control group and experimental group A, B were 0.84 ± 0.11,0.70 ± 0.16,0.82 ± 0.13; epididymis index: double distilled water control group A and B were 0.26 ± 0.08,0.24 ± 0.02,0.27 ± 0.05. Compared with double distilled water control group, there was no significant difference in epididymis index and testicular index in experimental group A and group B (P> 0.05). FSH level (U · L -1): The blank group, model group, double distilled water control group and experimental group A and B were 0.24 ± 0.04, 0.44 ± 0.01, 0.40 ± 0.00, 0.25 ± 0.04, 0.30 ± 0.05). The levels of GnRH, LH and T (ng · L -1) in the blank group were 1.90 ± 0.11, 4.30 ± 0.27 and 4.44 ± 0.11 respectively, and those in the model group were 2.33 ± 0.09, 7.99 ± 0.96 and 2.09 ± 0.40 ; Double distilled water control group were 2.33 ± 0.11,8.04 ± 0.36,2.00 ± 0.29; experimental group A were 1.91 ± 0.12,4.80 ± 0.70,4.07 ± 0.16; experimental group B were 1.99 ± 0.09,5.00 ± 0.29, 3.89 ± 0.26, the difference between the two groups was statistically significant (P <0.05). The relative expression levels of LHR mRNA in the blank group, model group, double distilled water control group and experimental A, B group were 1.00 ± 0.00,0.67 ± 0.03,0.66 ± 0.05,0.91 ± 0.04,0.90 ± 0.07 respectively; FSHR mRNA in blank The relative expression levels in the model group, the double distilled water control group and the experimental group A and group B were 1.00 ± 0.00,0.51 ± 0.05,0.50 ± 0.07,0.81 ± 0.06,0.80 ± 0.08, respectively, with significant difference between the two groups (All P <0.05). Sperm relative count (× 106): in the blank group, the model group, double distilled water control group and the experimental group A, B were 168.03 ± 11.25,79.17 ± 8.62,80.06 ± 10.13,143.39 ± 6.35,140.25 ± 9.41; sperm deformity The rate of sperm motility in the blank group, the model group, the double distilled water control group and the experimental group A and B were 6.03 ± 0.72, 20.57 ± 1.05, 21.07 ± 1.04, 6.20 ± 0.88 and 6.99 ± 1.02, respectively; Group, double distilled water control group and experimental group A, B were 87.13 ± 1.63,45.98 ± 3.02,48.09 ± 2.97,71.13 ± 4.02,69.08 ± 1.12. Compared with the control group, the relative counts, the activity rate and the deformity rate in the model group were significantly different (all P <0.05). Compared with the double distilled water control group, the sperm relative counts, The difference of deformity rate was statistically significant (all P <0.05). Conclusion Morinda officinalis plays a role in the repair of hypothalamic-pituitary-gonad axis injury in male adult SD rats under microwave irradiation.