论文部分内容阅读
目的采用乙睛-甲醇混合溶剂提取、气质联用分析法检测全血中奥氮平。方法针对奥氮平特性,在加入稳定剂硫代硫酸钠条件下采用乙睛-甲醇混合溶剂提取法,采用GC/MS分析法,对全血中的奥氮平进行定性、定量分析。并对提取介质、酸碱度、稳定剂种类及用量进行了考察。结果采用本文方法在奥氮平为1～400μg/mL浓度范围内呈良好的线性关系(r2=0.999 8,n=5),检出限为1.0ng,方法平均回收率可达66.5%(RSD=2.5%)。日内RSD为2.1%～3.9%,日间RSD为2.9%～4.5%。选择乙腈∶甲醇(V∶V=8∶2)混合溶剂进行液-液提取,稳定剂为300mg硫代硫酸钠。结论本文方法可用于全血中奥氮平检测,在相关案件检验中选择使用。
OBJECTIVE To study the extraction of olanzapine from whole blood with acetonitrile-methanol mixed solvent and GC-MS. Methods According to the characteristics of olanzapine, olanzapine in whole blood was qualitatively and quantitatively analyzed by GC / MS with acetonitrile - methanol mixed solvent extraction under the condition of adding sodium thiosulfate as stabilizer. And the extraction medium, pH, stabilizer type and dosage were investigated. Results The method showed a good linearity (r2 = 0.999 8, n = 5) in the range of 1 ~ 400 μg / mL with the detection limit of 1.0 ng. The average recovery was 66.5% (RSD = 2.5%). Day RSD was 2.1% ~ 3.9%, daytime RSD was 2.9% ~ 4.5%. Liquid-liquid extraction was carried out with a mixed solvent of acetonitrile: methanol (V: V = 8: 2), and the stabilizer was 300 mg sodium thiosulfate. Conclusion This method can be used for detection of olanzapine in whole blood, and is selected for use in testing of related cases.