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目的通过对不同浓度NaCl胁迫下膜荚黄芪种子萌发及幼苗生理特性的研究,寻找提高膜荚黄芪种子及幼苗在NaCl胁迫下抗性能力的途径。方法测定不同浓度NaCl胁迫下膜荚黄芪种子的发芽势(Gv)、发芽率(Gr)、相对发芽率、相对NaCl害率,并对幼苗的叶绿素量、可溶性蛋白质量、丙二醛(MDA)量、超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性进行测定。结果低浓度的NaCl胁迫对膜荚黄芪种子的萌发具有促进作用,当NaCl胁迫浓度>50 mmol/L时,随着NaCl浓度的增大,种子的Gv、Gr、相对发芽率逐渐降低,相对NaCl害率逐渐增大,对种子萌发有抑制作用。随着NaCl处理浓度的增大,膜荚黄芪幼苗中叶绿素量逐渐减少,可溶性蛋白质量也逐渐降低,二者都与NaCl处理浓度呈负相关;随着NaCl处理浓度的增加,幼苗中MDA量呈增加趋势,呈正相关;SOD、POD活性均不同程度地表现为先上升后下降的趋势,在75 mmol/L时达到最大值。结论膜荚黄芪具有一定的抗NaCl胁迫能力,具有向盐碱地引种驯化的潜力。
OBJECTIVE: To study the seed germination and physiological characteristics of Astragalus membranaceus under different concentrations of NaCl stress and to find ways to improve the resistance of Astragalus membranaceus seedlings and seedlings to NaCl stress. Methods The germination potential (Gv), germination rate (Gr), relative germination rate and relative NaCl damage rate of Astragalus membranaceus seedlings under different concentrations of NaCl stress were measured. The contents of chlorophyll, soluble protein, malondialdehyde (MDA) The activities of superoxide dismutase (SOD) and peroxidase (POD) were measured. Results Under low NaCl stress, the seed germination of Astragalus membranaceus was promoted. When the NaCl concentration was> 50 mmol / L, the relative germination rate of Gv, Gr decreased with the increase of NaCl concentration, Increasing the rate of injury, seed germination inhibition. With the increase of NaCl concentration, the amount of chlorophyll in Astragalus membranaceus decreased and the amount of soluble protein decreased gradually, both of them were negatively correlated with NaCl concentration. With the increase of NaCl concentration, the amount of MDA in seedlings was And the trend of increase was positively correlated. The activities of SOD and POD increased at first and then decreased with the increase of 75 mmol / L. Conclusion Astragalus membranaceus has the ability of resisting NaCl stress and has the potential of introduction and domestication to saline and alkali soil.