论文部分内容阅读
目的 探讨氯沙坦对自发性高血压大鼠 (SHR)脑组织血管紧张素Ⅰ型受体 (AT1 R)mRNA的表达及脑组织局部肾素 血管紧张素系统 (RAS)活性的影响。方法 6wk龄♂WKY和SHR各 1 6只分别随机分为氯沙坦用药组和生理盐水对照组 ,喂养 1 8wk后运用RT PCR法检测脑组织AT1 RmRNA的表达 ;放免法测定肾素活性 (RA)和血管紧张素Ⅱ(AngⅡ )的水平 ;紫外分光光度法测定血管紧张素转换酶活性 (ACEA)。结果 SHR组各部位脑组织AT1 RmRNA和血浆、下丘脑RA、AngⅡ、血清ACEA表达均高于WKY组(P <0 0 5) ;氯沙坦可降低各部位脑组织AT1 RmRNA的表达但升高血浆RA、AngⅡ ,对下丘脑RA、AngⅡ、ACEA则无明显影响。结论 SHR脑组织AT1 RmRNA的表达及循环和组织RAS活性增加可能与高血压的发生有关 ,氯沙坦可能通过降低脑组织AT1 RmRNA表达发挥脑保护作用
Objective To investigate the effects of losartan on the expression of angiotensin Ⅰ type 1 receptor (AT1R) mRNA and brain renin angiotensin system (RAS) activity in spontaneously hypertensive rats (SHR). Methods Sixteen WkY and WKY SHRs were randomly divided into losartan group and saline control group. The mRNA expression of AT1 mRNA in brain tissue was detected by RT-PCR after 18 weeks of feeding. The activity of renin (RA) ) And angiotensin Ⅱ (Ang Ⅱ) levels; UV spectrophotometry angiotensin converting enzyme activity (ACEA). Results AT1 R mRNA, RA, AngⅡ and ACEA in serum of the various tissues in SHR group were higher than those in WKY group (P <0.05). Losartan decreased the expression of AT1 mRNA in brain tissue of each group Plasma RA, Ang Ⅱ, on the hypothalamus RA, Ang Ⅱ, ACEA no significant effect. Conclusions The expression of AT1 R mRNA and the increase of circulatory and tissue RAS activity in SHR brain may be related to the occurrence of hypertension. Losartan may play a protective role in brain by reducing the expression of AT1 RmRNA