论文部分内容阅读
目的 探讨急性髓系白血病-M4、M5亚型的细胞遗传学、免疫表型特征。方法 应用染色体G显带、间期荧光原位杂交技术和流式细胞术对30例M4、M5患者进行遗传学分析和免疫表型检测。结果 染色体分析:30例M4、M5中共发现13例伴有11q2 3染色体异常,总发生率为4 3 3% ,其中M5a 1例、M5b 8例、M4b 4例,异常核型有6种:t (11;19) 4例;t(11;17) 2例;t(9;11) 2例;t(10 ;11) 1例;t(6 ;11) 1例;t(11;?) (q2 3;?) 1例;del(11)(q2 3) 2例。I-FISH检测MLL基因异常共13例阳性,其中M5 8例,M4 5例。免疫表型检测各种抗原阳性率依次为HLADR(92 % ) ,CD33(92 % ) ,CD6 4 (85 % ) ,CD11b(80 % ) ,CD15 (79% ) ,CD117(79% ) ,CD13(6 9% ) ,CD34(6 1% ) ,CD5 6 (38 5 % ) ,CD14 (31% ) ,CD3(14 % ) ,CD2 (7% ) ,CD7(7% )。结论 11q2 3异常在AML -M5 /M4中发生率高,该组患者高表达早期干祖细胞标志CD34、CD117、HLA -DR ,具有独特的遗传学、免疫学特点。
Objective To investigate the cytogenetic and immunophenotypic features of acute myeloid leukemia-M4 and M5 subtypes. METHODS: Genetic analysis and immunophenotyping were performed on 30 patients with M4 and M5 using chromosome G banding, interphase fluorescence in situ hybridization and flow cytometry. RESULTS: Chromosomal analysis: Thirteen patients with M4 and M5 were found to have chromosome abnormalities of 11q2 3 in 13 cases. The total incidence rate was 433%. Among them, M5a was 1 case, M5b was 8 cases, and M4b was 4 cases. There were 6 abnormal karyotypes: t (11; 19) 4 cases; t (11; 17) 2 cases; t (9; 11) 2 cases; t (10; 11) 1 case; t (6; 11) 1 case; t (11; ?) (q2 3;?) 1 case; del(11)(q2 3) 2 cases. I-FISH detected 13 cases of MLL gene abnormalities, including 8 cases of M5 and 5 cases of M4. The positive rate of various antigens detected by immunophenotype was HLADR (92%), CD33 (92%), CD6 4 (85%), CD11b (80 %), CD15 (79%), CD117 (79%), CD13 ( 6 9%), CD34 (6 1%), CD5 6 (38.5 %), CD14 (31%), CD3 (14%), CD2 (7%), CD7 (7%). Conclusion The abnormal rate of 11q2 3 is high in AML-M5/M4. This group of patients has high expression of early markers of progenitor cells CD34, CD117 and HLA-DR, and has unique genetic and immunological characteristics.