建立了食品中沙门菌和单增李斯特菌的多重PCR-芯片电泳快速检测方法。根据沙门菌和单增李斯特菌的特征基因合成2对特异性引物,优化聚合酶链反应(PCR)体系,采用芯片毛细管电泳快速检测食品中上述2种致病菌的多重PCR扩增产物。在优化的实验条件下,6 min内即可完成沙门菌和单增李斯特菌的同时检测;迁移时间的日内精密度为0.20%～1.7%,日间精密度3.7%～4.5%。 A multiplex PCR-chip rapid detection method for detecting Salmonella and Listeria monocytogenes in food was established. Two pairs of specific primers were synthesized based on the characteristic genes of Salmonella and Listeria monocytogenes, and the polymerase chain reaction (PCR) system was optimized. The multiplex PCR amplification products of the above two kinds of pathogens in food were detected by chip capillary electrophoresis. Under optimized experimental conditions, simultaneous detection of Salmonella and Listeria monocytogenes could be completed in 6 min. The intra-day precision of migration time was 0.20% -1.7% and the intra-day precision was 3.7% -4.5%.