论文部分内容阅读
目的研究全反式维A酸(ATRA)诱导肺鳞癌细胞株A2凋亡作用并初步探讨其作用机制,寻找肺癌治疗的新途径。方法体外培养A2细胞,随机分为两组,实验组加ATRA使其终浓度为5μmol/L,对照组加入二甲亚砜使其终浓度为0.1%,继续培养48 h后用流式细胞仪技术分别检测Rb、p53基因表达率,同时用DNA凋亡分析法检测肿瘤细胞凋亡发生率,研究三者之间的相关关系。结果A2细胞实验组中细胞凋亡发生率显著增高(P<0.01),Rb和p53基因表达率显著增强(P<0.01)。A2细胞中凋亡发生率与Rb基因表达率之间正相关(P<0.05),与p53基因表达率之间亦呈正相关关系(P<0.05)。结论ATRA可能通过上调Rb和p53基因表达途径使A2细胞阻滞在G0/G1期,进而诱导肺癌细胞A2凋亡。
Objective To study the apoptosis of lung squamous carcinoma cell line A2 induced by all-trans-retinoic acid (ATRA) and to explore its mechanism of action, in order to find a new approach for the treatment of lung cancer. Methods A2 cells were cultured in vitro and randomly divided into two groups. The experimental group was treated with ATRA to a final concentration of 5 μmol/L, and the control group was treated with dimethyl sulfoxide to a final concentration of 0.1%. The culture was continued for 48 h before the use of flow cytometry. Techniques were used to detect the expression rates of Rb and p53 genes. At the same time, DNA apoptosis assay was used to detect the incidence of tumor cell apoptosis and the relationship between the three was studied. Results The apoptosis rate of A2 cells in the experimental group was significantly higher (P<0.01), and the expression rate of Rb and p53 genes was significantly increased (P<0.01). There was a positive correlation between the rate of apoptosis in A2 cells and the expression rate of Rb gene (P<0.05) and a positive correlation with the rate of p53 gene expression (P<0.05). Conclusion ATRA may induce A2 cell apoptosis in G0/G1 phase by up-regulating Rb and p53 gene expression pathways, which in turn induce apoptosis in lung cancer cell A2.