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虎眼万年青具有很高的药用价值和经济价值,鳞茎是其主要的药用和繁殖器官,然而虎眼万年青鳞茎的分子生物学信息严重缺乏成为制约其研究生产的瓶颈。为获得虎眼万年青鳞茎的转录组信息,应用高通量测序技术对虎眼万年青鳞茎进行测序分析。采用His4000测序平台共获得8.2 G原始数据,经过整理分析后,获得7.6 G有效数据和121 223条Unigene;经过与NR、GO、COG及KEGG等数据库进行比较分析后,56 374条Unigene获得NR数据库的注释,39 263条Unigene获得GO数据库的注释,12 388条Unigene获得COG功能注释,5 405条Unigene参与了新陈代谢途径,2 477条Unigene参与了次生代谢途径,166条Unigene参与了萜类的合成,195条Unigene参与了生物碱的合成,295条Unigene作为转录因子参与新陈代谢过程调控。研究结果为采用生物技术手段提高虎眼万年青的药用成分的含量及改善其园艺性状提供了分子数据。
Ornithogalum evergreen has high medicinal value and economic value. Bulb is its main medicinal and reproductive organs. However, the serious lack of molecular biology information of Ornithogalum bulbum has become the bottleneck restricting its production. In order to obtain the transcriptome information of Ornithogalum bulbum, we sequenced the bulb of Ornithogalum evergreen using high-throughput sequencing. A total of 8.2 G raw data was obtained using the His4000 sequencing platform. After finishing the analysis, 7.6 G valid data and 121 223 Unigene were obtained. After comparing with databases such as NR, GO, COG and KEGG, 56 374 Unigene databases were obtained , 39 263 Unigene obtained GO database notes, 12 388 Unigene obtained COG functional annotations, 5 405 Unigene involved in metabolic pathway, 2 477 Unigene involved in secondary metabolic pathway, 166 Unigene involved in terpenoids Synthesis, 195 Unigene involved in the synthesis of alkaloids, 295 Unigene as a transcription factor involved in the regulation of metabolic processes. The results provide molecular data for improving the content of medicinal components and improving the horticultural traits of Ornithogalum evergreen by biotechnology.