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本研究利用大鼠离体心脏模型观察了黄芪对心肌缺血—再灌注后氧自由基及心肌细胞、亚细胞结构的影响。全心缺血50min,再灌注25min后,缺血—再灌注组心肌SOD活性降低,MDA含量增高,心肌细胞水肿明显,肌纤维断裂,线粒体损伤严重,糖原颗粒明显减少;而缺血前15min给予黄芪配制的灌注液(5mg/ml)灌注心脏至再灌注后15min,则明显保护了再灌注25min时的心肌,其心肌SOD活性增高,MDA含量下降,与缺血—再灌注组相比有显著性差异(P<0.05及P<0.01),并且心肌细胞水肿不明显,无明显肌纤维断裂,线粒体保护良好,糖原颗粒较缺血—再灌注组明显增多。黄芪保护心肌细胞及亚细胞结构的作用可能与其抗氧自由基作用有关。
In this study, the effects of Astragalus membranaceus on oxygen free radicals, cardiomyocytes, and subcellular structures after myocardial ischemia-reperfusion were observed in isolated rat heart models. After 50 min of ischemic ischemia and 25 min of reperfusion, the activity of SOD in myocardium of ischemic-reperfusion group was decreased, MDA content was increased, edema of cardiomyocytes was obvious, myofibers were broken, mitochondrial damage was severe, and glycogen granules were significantly reduced; however, it was given 15 min before ischemia. Perfusion of astragalus (5mg/ml) perfused into the heart to 15min after reperfusion, obviously protected myocardium at the time of reperfusion 25min, its myocardial SOD activity increased, MDA content decreased, compared with ischemia-reperfusion group significantly The differences were statistically significant (P<0.05 and P<0.01), and myocardial cell edema was not obvious, no muscle fiber breakage was observed, mitochondria were well protected, and glycogen granules were significantly increased compared with the ischemia-reperfusion group. The protective effect of Astragalus on cardiomyocytes and subcellular structures may be related to its anti-oxygen free radical action.