论文部分内容阅读
目的 :评价重组人红细胞生成素 (rHuEPO)、重组人粒细胞 -巨噬细胞集落刺激因子 (rHuGM CSF) ,对SD大鼠芥子气全身中毒的治疗作用。方法 :本实验以SD大鼠为动物模型 ,4mg/kg体重芥子气皮下注射为中毒剂量。治疗组动物在中毒后 0 .5h即皮下注射治疗药物 ,其中rHuEPO的剂量为 30 0IU/kg体重 ,第 1天和第 3天各 1次 ;rHuGM CSF的剂量为15 μg/kg体重 ,连续应用 5d。通过观察 1周内动物腹泻率、死亡率、体重变化、血常规指标变化、骨髓细胞增生程度和细胞学分类等来评价药物疗效。结果 :和中毒对照组相比 ,药物治疗组的腹泻率、死亡率、动物体重、血常规指标以及骨髓细胞学分类等均无显著差异。但治疗组在中毒后第 8天的血常规中红细胞 (RBC)和血红蛋白 (HGB)已上升到和正常对照组无明显差异 ,而中毒对照组却仍然显著低于正常对照组。另外 ,虽然统计学上无显著差异 ,但骨髓细胞分类显示治疗组中单核细胞系比例和红细胞系比例也较中毒对照组有升高趋势。结论 :SD大鼠芥子气中毒后 ,只应用rHuEPO和rHuGM CSF进行药物治疗 ,虽然能一定程度上刺激骨髓红细胞系和单核细胞系的生长 ,但对其他细胞系的刺激作用不明显 ,而且反而可能由于其副作用使动物的腹泻率和死亡率升高。因此在芥子气全身中毒的综合治疗方案中 ,
Objective: To evaluate the therapeutic effect of recombinant human erythropoietin (rHuEPO) and recombinant human granulocyte-macrophage colony stimulating factor (rHuGM CSF) on systemic poisoning of SD rat mustard gas. Methods: In this experiment, SD rats as animal model, 4 mg / kg body weight mustard subcutaneously injected as toxic dose. The animals in the treatment group were treated subcutaneously 0.5 h after poisoning, with rHuEPO at a dose of 30 IU / kg body weight, on day 1 and 3, and rHuGM CSF at 15 μg / kg body weight for continuous use 5d. The efficacy of the drug was evaluated by observing the animal diarrhea rate, mortality, body weight changes, changes in blood routine, bone marrow hyperplasia and cytological classification within 1 week. Results: Compared with the poisoned control group, there was no significant difference in the diarrhea rate, mortality rate, body weight, blood index and the myelocytic classification of the drug treatment group. However, the RBC and hemoglobin (HGB) of the treated group rose to the level of the normal control group on the 8th day after poisoning, while the poisoned control group was still significantly lower than the normal control group. In addition, although there was no statistically significant difference, the classification of myeloid cells showed that mononuclear cell lines and red blood cell lines in the treated group also showed an increased tendency compared with the poisoned control group. CONCLUSIONS: After treatment with mustard gas in rats, only rHuEPO and rHuGM CSF should be used for drug treatment. Although it can stimulate the growth of bone marrow erythrocyte and monocyte cell lines to some extent, the stimulation to other cell lines is not obvious, Animal’s diarrhea and mortality are increased due to its side effects. Therefore, mustard gas poisoning in the comprehensive treatment program,