Evaluation of the disc diffusion method with a comparison study for fluconazole susceptibility of C

来源 :Chinese Medical Journal | 被引量 : 0次 | 上传用户:jldxxys
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To performance susceptibility testing of antifungal agents Due to the increasing number of resistant strains, susceptibility testing of antifungal agents is gaining importance Methods We compared the results of standard macrotube dilution reference method with two different microdilution methods, as well as the disc diffusion method in order to test the susceptibility of 150 Candida strains to fluconazole Results Overall correlation between microdilution and macrodilution methods was 86% It was 91% between the Minimal Inhibitory Concentrations obtained from macrodilution and disc diffusion zone diameters Conclusion The disc diffusion test was evaluated as a low-cost, reproducible, and efficient way of assessing the in vitro susceptibility of Candida strains to fluconazole To performance susceptibility testing of antifungal agents Due to the increasing number of resistant strains, susceptibility testing of antifungal agents is gaining in importance methods Methods for comparing the results of standard macrotube dilution reference methods with two different microdilution methods, as well as the disc diffusion method in order to test the susceptibility of 150 Candida strains to fluconazole Results Overall correlation between microdilution and macrodilution methods was 86% It was 91% between the Minimal Inhibitory Concentrations obtained from macrodilution and disc diffusion zone diameters Conclusion The disc diffusion test was evaluated as a low-cost , reproducible, and efficient way of assessing the in vitro susceptibility of Candida strains to fluconazole
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