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本研究以水稻籼粳杂交 (窄叶青 8号×京系 17) F1经花药培养 ,产生的双单倍体 ( DH)群体和应用该群体已构建的分子图谱为基础。采用 QTL区间作图法对 5个剑叶性状进行定位分析 ,结果表明 ,在 DH群体中 ,剑叶长、宽、长宽比和叶面积呈连续分布 ,受微效多基因控制 ,并且各性状均存在一定数量的超亲遗传类型。 4个性状共检测出 13个 QTL s位点 ,分布于 6条染色体 ;这些 QTL s对相应性状的贡献率介于 8.7%～ 18.5%之间。其中在第 4和第 8染色体上检测到控制剑叶长的 2个 QTL -s;在第 1,3,8和 12染色体上分别检测到 4个 QTL s与叶宽有关 ;影响剑叶长宽比的 5个 QTL s分别定位于第 1,3和 7染色体上 ;控制叶面积的两个 QTL s分别与控制叶长的两个 QTLs一致。叶片卷曲受两对互补的主效基因 rl- 1和 rl- 7控制 ,分别位于第 1和第 5染色体 ,其中卷叶 DH系的基因型为 rl-1rl- 1rl- 7rl- 7
This study was based on the double haploid (DH) population from rice indica-japonica hybrid (Narrow Yeqing 8 × Jing-17) F1 cultured by anther culture and the constructed molecular map of the population. The QTL interval mapping method was used to locate and analyze the five flag leaf traits. The results showed that in the DH population, the length, width, aspect ratio and leaf area of flag leaves were continuously distributed and controlled by the micro-multi-gene, There is a certain number of super-progeny types. Thirteen QTLs were detected in four traits and distributed on six chromosomes. The contribution rate of these QTLs to the corresponding traits ranged from 8.7% to 18.5%. Two QTLs controlling the length of flag leaf were detected on chromosomes 4 and 8. Four QTLs were detected on chromosomes 1, 3, 8 and 12, respectively, Five QTLs were located on chromosomes 1, 3 and 7, respectively. Two QTLs controlling leaf area were consistent with the two QTLs controlling leaf length respectively. Leaf curl is controlled by two pairs of complementary major genes, rl-1 and rl-7, located on chromosomes 1 and 5, respectively, and the genotype of leaf curl DH line is rl-1rl-1rl-7rl-7