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近来有人对许多天然存在于食品中的黄酮类化合物进行了体外致突致/癌试验,有几种已被发现在体外能引起致突变作用。这一结果提示含黄酮类成分的食物和饮料可能有致癌的危险。槲皮黄酮是最常见并在体外试验中致突变性最强的一种黄酮醇类物质。本研究采用微核和宿主间介的体内试验测定槲皮黄酮的致突变性,为了解槲皮黄酮的吸收情况,先进行迁移试验并据此设计致突变试验。实验均使用10周龄小鼠,经口给予槲皮黄酮水或PBS悬液。迁移试验:为研究槲皮黄酮在消化道的分布,给予动物~(51)Cr标记的槲皮黄酮,在给药后不同时间处死,取出胃肠道并分成9段分别测
Several recent in vitro-induced / carcinogenic tests of flavonoids found naturally in food have been reported to cause in vitro mutagenic effects. This result suggests that foods and beverages containing flavonoids may be carcinogenic. Quercetin is the most common and in vitro mutagenicity strongest flavonols. In this study, the in vitro mutagenicity of quercetin was determined by the in vitro assay mediated by micronuclei and the host. To understand the absorption of quercetin, migration experiments were carried out and the mutagenicity test was designed accordingly. 10-week-old mice were used for oral administration of quercetin water or PBS suspension. Migration test: To study the distribution of quercetin in the digestive tract, animals were given Cr (51) Cr-labeled quercetin and sacrificed at different times after administration. The gastrointestinal tract was removed and divided into 9 segments