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目的利用基因芯片技术分析比较基因敲入(KIMAP)和转基因(TGMAP)小鼠前列腺癌模型。方法对于来自KIMAP和TGMAP的肿瘤标本进行了基因芯片分析和比较。结果基因芯片分析证明,在KIMAP中免疫应答基因高表达,而TGMAP晚期肿瘤(26～32周)中与神经内分泌肿瘤(NE)分化相关的基因占主体,而NE肿瘤在人类前列腺癌中少于10%。人类前列腺癌的几个肿瘤标记物基因在KIMAP中均可检测到(在TGMAP中缺乏),包括hepsin、maspin、Nkx3.1、CD10和PSP94等。结论由于KIMAP模型成功地模仿了人类前列腺癌特征,在前列腺癌临床前期研究中具有潜在的应用价值。
Objective To analyze gene knock-in (KIMAP) and transgenic (TGMAP) mouse models of prostate cancer using gene chip technology. Methods Gene chip analysis and comparison of tumor samples from KIMAP and TGMAP. Results Gene microarray analysis demonstrated that the immune response genes are highly expressed in KIMAP whereas the genes associated with neuroendocrine neoplasia (NE) differentiation are predominant in advanced TGMAP tumors (26-32 weeks), whereas NE tumors are less common in human prostate cancer than 10%. Several tumor marker genes of human prostate cancer are detectable in KIMAP (lacking in TGMAP) including hepsin, maspin, Nkx3.1, CD10 and PSP94. Conclusion Because KIMAP model successfully mimics the characteristics of human prostate cancer, it has potential value in the preclinical study of prostate cancer.